Evidence for cell proliferation in the sheep brain and its down-regulation by parturition and interactions with the young
Evidence for cell proliferation in the sheep brain and its down-regulation by parturition and interactions with the young
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DOI：
10.1016/j.yhbeh.2010.07.006
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发表时间：
2010-11-01
影响因子：
3.5
通讯作者：
Levy, F.
中科院分区：
文献类型：
--
作者：
Brus, M.;Meurisse, M.;Levy, F.
新神经元的产生在脑室下区(SVZ)和海马齿状回(DG)中持续存在，并受到内分泌和社会因素的影响。在绵羊中，分娩与建立基于嗅觉学习的与幼羊的选择性联系有关。存在这样一种可能性，即分娩时内分泌的变化以及与幼崽的相互作用调节了神经原区的细胞增殖。在本研究中，我们首先研究了绵羊体内细胞增殖的存在。细胞增殖标记物5-溴-2‘-脱氧尿苷(BrdU)标记的新生细胞位于SVZ、主嗅球(MOB)和DG，并与另一有丝分裂标志物Ki-67完全共定位。40%至50%的BrdU标记细胞含有GFAP，提示存在神经干细胞。其次，在与羔羊有或没有相互作用的情况下分娩2天，与不怀孕相比，下调了3个增殖部位的BrdU标记细胞的数量。另一项对照提供的证据表明，这种效应是产后早期特有的：发情与雄性动物的互动不会影响细胞增殖。我们的结果首次描述了SVZ、DG和成年绵羊群体中神经细胞增殖的特征。我们假设，在产后早期观察到的细胞增殖下调可能通过促进较早出生的神经元的存活和整合来促进与母亲行为相关的嗅觉、感知和记忆需求。(C)2010 Elsevier Inc.保留所有权利。
Production of new neurons continues throughout life in the subventricular zone (SVZ) and the dentate gyrus (DG) of the hippocampus and is influenced by both endocrine and social factors. In sheep parturition is associated with the establishment of a selective bond with the young based on an olfactory learning. The possibility exists that endocrine changes at parturition together with interactions with the young modulate cell proliferation in the neurogenic zones. In the present study, we first investigated the existence of cell proliferation in sheep. Newly born cells labeled by the cell proliferation marker 5-bromo-2'-deoxyuridine (BrdU) were found in the SVZ, the main olfactory bulb (MOB) and the DG and completely co-localized with Ki-67, another mitotic marker. Forty to 50% of the BrdU-labeled cells contained GFAP suggestive of the presence of neural stem cells. Secondly, parturition with or without interactions with the lamb for 2 days, down-regulated the number of BrdU-labeled cells in the 3 proliferation sites in comparison to no pregnancy. An additional control provided evidence that this effect is specific to early postpartum period: estrus with interactions with males did not affect cell proliferation. Our results provide the first characterization of neural cell proliferation in the SVZ, the DG and unexpectedly in the MOB of adult sheep. We hypothesize that the down-regulation of cell proliferation observed in the early postpartum period could facilitate the olfactory perceptual and memory demands associated with maternal behavior by favouring the survival and integration of neurons born earlier. (C) 2010 Elsevier Inc. All rights reserved.