Light-dependent conformational change of neoxanthin in a siphonous green alga, Codium intricatum, revealed by Raman spectroscopy
Light-dependent conformational change of neoxanthin in a siphonous green alga, Codium intricatum, revealed by Raman spectroscopy
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DOI：
10.1007/s11120-014-0011-y
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发表时间：
2014-05
影响因子：
3.7
通讯作者：
Chiasa Uragami;Denise Galzerano;A. Gall;Yusuke Shigematsu;Maïwen Meisterhans;N. Oka;M. Iha;R. Fujii;B. Robert;H. Hashimoto
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文献类型：
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作者：
Chiasa Uragami;Denise Galzerano;A. Gall;Yusuke Shigematsu;Maïwen Meisterhans;N. Oka;M. Iha;R. Fujii;B. Robert;H. Hashimoto
管形绿色藻类是深海绿色藻类的一种，呈橄榄褐色，利用蓝绿色光进行光合作用。本文报道了一种管形绿色松藻Codium（C.）intertricatum，分离自日本的冲绳县，并建立了丝状形式的克隆藻类培养物。主要的捕光天线类似于高等植物中发现的三聚体LHC II，但C。intertricatumcomplex含有一种不寻常的羰基类胡萝卜素管黄质。优化培养条件以在完整的冻干丝状体中实现高的管黄质含量。有趣的是，类胡萝卜素的组成是不同的，当培养在高辐射：全transneoxanthin积累除了正常的9′-顺式在整个细胞提取物。共振拉曼光谱的完整的丝状体，在高和低光条件下培养，证实了所有transneoxanthin在高辐照条件下的积累。将讨论全反式新黄质存在的合理功能与对抗高光胁迫的调节的关系。
Siphonous green algae, a type of deep-sea green algae, appear olive drab and utilize blue–green light for photosynthesis. A siphonous green alga,Codium (C.) intricatum, was isolated from Okinawa prefecture in Japan, and a clonal algal culture in filamentous form was established. The major light-harvesting antenna was analogous to the trimeric LHCII found in higher plants, but theC. intricatumcomplex contained an unusual carbonyl carotenoid siphonaxanthin. Culture conditions were optimized to achieve high siphonaxanthin content in intact lyophilized filamentous bodies. Interestingly, the carotenoid composition was different when cultured under high irradiance: all-transneoxanthin was accumulated in addition to the normal 9′-cisform in whole cell extract. Resonance Raman spectra of intact filamentous bodies, cultured under high- and low-light conditions, confirmed the accumulation of all-transneoxanthin under high irradiance conditions. A plausible function of the presence of all-transneoxanthin will be discussed in relation to the regulation against high light stress.