Validation of a device for the active manipulation of the tumor microenvironment during intravital imaging.
Validation of a device for the active manipulation of the tumor microenvironment during intravital imaging.
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验证活体成像期间主动操纵肿瘤微环境的装置。
DOI：
10.1080/21659087.2016.1182271
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Condeelis,JohnS
中科院分区：
文献类型：
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作者：
Williams,JamesK;Entenberg,David;Wang,Yarong;Avivar-Valderas,Alvaro;Padgen,Michael;Clark,Ashley;Aguirre-Ghiso,JulioA;Castracane,James;Condeelis,JohnS
肿瘤微环境被认为在肿瘤细胞的行为及其向转移的进展中起重要作用。然而，直接操纵肿瘤微环境的工具，并在体内以真实的时间以单细胞分辨率成像这种操纵的结果，是缺乏的。我们在这里描述了一种方法，通过使用可植入的诱导纳米活体装置（iNANIVID）和在单细胞分辨率下的结果在体内可视化的直接，局部操作的微环境参数。作为概念证明，我们向肿瘤细胞提供持续剂量的EGF，同时活体成像其趋化反应，并在实体瘤中的特定微环境中局部诱导缺氧。
The tumor microenvironment is recognized as playing a significant role in the behavior of tumor cells and their progression to metastasis. However, tools to manipulate the tumor microenvironment directly, and image the consequences of this manipulation with single cell resolution in real timein vivo, are lacking. We describe here a method for the direct, local manipulation of microenvironmental parameters through the use of an implantable Induction Nano Intravital Device (iNANIVID) and simultaneousin vivovisualization of the results at single-cell resolution. As a proof of concept, we deliver both a sustained dose of EGF to tumor cells while intravital imaging their chemotactic response as well as locally induce hypoxia in defined microenvironments in solid tumors.