Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg2+ ion
Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg2+ ion
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基于小檗碱的无标记荧光适体传感器超灵敏检测 Hg2 离子的策略
DOI：
10.1016/j.saa.2018.06.058
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Dong Chuan
中科院分区：
文献类型：
--
作者：
Song Xiuli;Fu Baochun;Lan Yifeng;Chen Yanxia;Wei Yanli;Dong Chuan
构建了一种无标记的荧光适配体平台，并以黄连素为荧光探针，以Hg 2+离子适配体为识别元件，建立了一种简便、快速检测水溶液中Hg 2+离子的方法。考察了小檗碱浓度、Hg 2+浓度、Hg 2 +-适配体浓度、pH值、反应时间等因素对Hg 2 +-适配体的影响。在最佳实验条件下，Hg 2+离子在0.1 ~ 10.0 μM范围内，荧光强度变化呈线性关系，检测限为7.7 nM（S/N = 3）。所制备的无标记荧光适体传感器可方便、有效地用于血清和自来水中Hg ~（2+）离子的分析，回收率为96.0%~ 105.7%。总之，本研究提供了一个简单、经济、稳定的检测系统，用于在恶劣条件下检测Hg ~（2+）离子。
A label-free fluorescent aptasensing platform was fabricated and a simple and rapid method to detect Hg2+ion in aqueous solution was put forward by means of berberine and Hg2+ion-aptamer are as the fluorescence probe and the recognition element, respectively. Various factors including the concentration of berberine, Hg2+ion and Hg2+ion-aptamer, pH effect and the reaction time were investigated in detail. Under the optimal experimental conditions, in the sensing system, the fluorescence intensity changes displayed a calibration response for Hg2+ion in the range of 0.1 μM to 10.0 μM and the detection limit was of 7.7 nM (S/N = 3). The fabricated label-free fluorescence aptasensor is not only conveniently but also effectively applicable used for analysis of Hg2+ion in blood serum and tap water samples and the recovery range is of 96.0%–105.7%. In brief, this study offers an easy, economical and stable assay system for detecting Hg2+ion in rough condition.