A Class of Rigid Linker-bearing Glucosides for Membrane Protein Structural Study.
A Class of Rigid Linker-bearing Glucosides for Membrane Protein Structural Study.
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一类用于膜蛋白结构研究的刚性连接基糖苷。
DOI：
10.1039/c5sc02900g
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发表时间：
2016
期刊：
影响因子：
8.4
通讯作者：
Chae,PilSeok
中科院分区：
文献类型：
--
作者：
Sadaf,Aiman;Mortensen,JonasS;Capaldi,Stefano;Tikhonova,Elena;Hariharan,Parameswaran;deCastroRibeiro,Orquidea;Loland,ClausJ;Guan,Lan;Byrne,Bernadette;Chae,PilSeok
膜蛋白是与水介质的极性环境不相容的两亲性生物大分子。传统的去污剂封装膜蛋白的疏水表面，使它们能够存在于水溶液中。通过去污剂胶束稳定的膜蛋白用于结构和功能分析。尽管有大量的去垢剂可供使用，但只有少数试剂能够充分有效地维持膜蛋白的完整性以实现成功的结晶。在本研究中，我们描述了一类新型合成两亲物，具有支链尾基和三葡萄糖苷头基。这些头部和尾部基团通过酰胺或醚键通过三（羟甲基）氨基甲烷（TRIS）或新戊二醇（NPG）连接体连接，分别产生TRIS衍生的三葡萄糖苷（TDT）和NPG衍生的三葡萄糖苷（NDT）。与传统去污剂相比，此类成员赋予靶膜蛋白更强的稳定性。由于新试剂的直接合成及其对一系列膜蛋白的有利影响，这些试剂应该对膜蛋白科学具有广泛的适用性。
Membrane proteins are amphipathic bio-macromolecules incompatible with the polar environments of aqueous media. Conventional detergents encapsulate the hydrophobic surfaces of membrane proteins allowing them to exist in aqueous solution. Membrane proteins stabilized by detergent micelles are used for structural and functional analysis. Despite the availability of a large number of detergents, only a few agents are sufficiently effective at maintaining the integrity of membrane proteins to allow successful crystallization. In the present study, we describe a novel class of synthetic amphiphiles with a branched tail group and a triglucoside head group. These head and tail groups were connected via an amide or ether linkage by using a tris(hydroxylmethyl)aminomethane (TRIS) or neopentyl glycol (NPG) linker to produce TRIS-derived triglucosides (TDTs) and NPG-derived triglucosides (NDTs), respectively. Members of this class conferred enhanced stability on target membrane proteins compared to conventional detergents. Because of straightforward synthesis of the novel agents and their favourable effects on a range of membrane proteins, these agents should be of wide applicability to membrane protein science.