Effects of polyethylene on macrophages
Effects of polyethylene on macrophages
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DOI：
10.1002/jor.1100150108
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发表时间：
1997-01-01
影响因子：
2.8
通讯作者：
Gonzales, JB
中科院分区：
文献类型：
--
作者：
Horowitz, SM;Gonzales, JB
开发了一种在体外将巨噬细胞暴露于聚乙烯的系统。巨噬细胞单独暴露于这些颗粒会导致肿瘤坏死因子 α 和前列腺素 E(2) 的释放。与成骨细胞共培养的巨噬细胞暴露于聚乙烯颗粒会增加前列腺素 E(2) 的释放，并导致白细胞介素 6 的释放。将放射性标记的颅盖与来自单独暴露于聚乙烯颗粒或与成骨细胞共培养的颗粒的巨噬细胞的条件培养基一起孵育，导致通过Ca-45的释放反映的骨吸收。与帕米膦酸盐一起孵育可有效抑制条件培养基刺激的巨噬细胞的吸收，巨噬细胞单独暴露于这些颗粒或与成骨细胞共培养。这证明帕米膦酸盐或其他双膦酸盐可有效抑制植入物/骨界面处的骨吸收，这与巨噬细胞对颗粒聚乙烯的反应有关。有必要进一步研究帕米膦酸盐或其他双膦酸盐在治疗无菌性松动中的可能用途。
A system was developed to expose macrophages to polyethylene in vitro. Exposure of macrophages to these particles in isolation led to the release of tumor necrosis factor alpha and prostaglandin E(2). Exposure of macrophages in co-culture with osteoblasts to polyethylene particles increased the release of prostaglandin E(2) and also led to the release of interleukin-6. Incubation of radiolabelled calvariae with conditioned medium from macrophages exposed to polyethylene particles alone or to particles in co-culture with osteoblasts led to bone resorption reflected by release of Ca-45. Incubation with pamidronate was effective in inhibiting resorption stimulated by conditioned medium from macrophages exposed to these particles alone or in co-culture with osteoblasts. This demonstrates that pamidronate, or other bisphosphonates, may be effective in inhibiting bone resorption at the implant/bone interface in association with the macrophage response to particulate polyethylene. Further investigation into the possible use of pamidronate or other bisphosphonates in the treatment of aseptic loosening is warranted.