Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.
复制标题
青蛙细胞中兔血红蛋白的合成：青蛙卵母细胞中网织红细胞 9s RNA 的翻译。
DOI：
10.1016/0022-2836(71)90207-5
复制
发表时间：
1971
影响因子：
5.6
通讯作者：
J. Gurdon
中科院分区：
文献类型：
--
作者：
C. Lane;G. Marbaix;J. Gurdon
将从兔网织红细胞制备的4枚S+5枚S核糖核酸、9枚S核糖核酸、18枚S核糖核酸、28枚S核糖体和多聚核糖体注射到非洲爪蛙活卵母细胞中。注射9只S核糖核酸或多聚核糖体后，才形成血红蛋白样物质。用凝胶过滤、羧甲基纤维素层析、丙烯酰胺凝胶电泳法和胰蛋白肽离子交换层析法鉴定血红蛋白。9 S核糖核酸对类珠蛋白物质的形成有促进作用，无论有没有注射血红素，9 S核糖核酸在注入活细胞时都比较稳定，具有血红蛋白信使的性质。信使的翻译不需要网织红细胞特有的因子，卵母细胞的翻译机制将接受来自完全不同细胞类型的信使RNA，来自另一个物种。在装配和合成的所有阶段，血红蛋白分子在卵母细胞中都相当稳定。
4 s + 5 s RNA, 9 s RNA, 18 s RNA, 28 s RNA and polyribosomes, all prepared from rabbit reticulocytes, were injected into living oocytes of the frogXenopus laevis. Only after injection of 9 s RNA or polyribosomes was haemoglobin-like material formed. Haemoglobin was identified by various criteria: gel filtration, carboxymethyl cellulose chromatography, acrylamide gel electrophoresis and ion exchange chromatography of tryptic peptides. 9 s RNA promoted the formation of globin-like material, whether injected with haemin or without haemin.It is concluded that when injected into a living cell, the 9 s RNA is fairly stable and has the properties of a haemoglobin messenger. The messenger requires no reticulocyte-specific factors for translation, and the translational machinery of the oocyte will accept the messenger RNA from a totally different cell type, from another species. At all stages of assembly and synthesis haemoglobin molecules are reasonably stable in oocytes.