A virulence attenuated amoebapore-less mutant of Entamoeba histolytica and its interaction with host cells
A virulence attenuated amoebapore-less mutant of Entamoeba histolytica and its interaction with host cells
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DOI：
10.1016/s0020-7519(03)00268-6
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发表时间：
2003-12-01
影响因子：
4
通讯作者：
Mirelman, D
中科院分区：
文献类型：
--
作者：
Bujanover, S;Katz, U;Mirelman, D
溶组织内阿米巴是一种引起阿米巴病的原生动物寄生虫，是一种专属于人类的病原体，因此开发疫苗可以有效地影响疾病的传播。最近，我们开发了一个遗传修饰的无毒菌株，称为G3，从强毒E。溶组织菌HM-1：IMSS。新菌株缺乏重要的毒力因子，阿米巴孔-A。我们目前研究的目的是调查减毒株的无毒性，以及检查这些滋养体作为活疫苗的潜在候选者的抗原性和免疫原性反应。进行功能测定以确定G3菌株的毒力行为。将该行为与强毒株HM-1：IMSS和非强毒株Rahman进行了比较。Western印迹法证实E.溶组织G3菌株，并证明它对其他毒力因子的存在没有影响。这两组试验的结果证明，G3菌株与无毒力的Rahman菌株表型相似，而与有毒性的HM-1：IMSS在抗原性上相同，除了缺乏阿米巴孔-A蛋白。与假免疫仓鼠相比，G3滋养体腹膜内免疫仓鼠产生IgG抗HM-1：IMSS抗体。体液反应水平是可变的，在引入这种新菌株作为疫苗之前必须进行进一步的测试。(C)2003年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Entamoeba histolytica, the protozoan parasite which causes amoebiasis, is an exclusively human pathogen so developing a vaccine could effectively impact the spread of the disease. Recently we developed a genetically modified avirulent strain, termed G3, from the virulent E. histolytica strain HM-1:IMSS. The new strain lacks the important virulence factor, the amoebapore-A. The objective of our current study was to investigate the avirulence of the attenuated strain as well as to examine the antigenic and immunogenic responses of these trophozoites as potential candidates for a live vaccine. Functional assays were conducted to characterise the virulent behaviour of the G3 strain. This behaviour was compared to the virulent strain HM-1: IMSS and the non-virulent strain Rahman. Western blots were conducted to confirm the lack of amoebapore-A in the E. histolytica G3 strain and to demonstrate that it had no influence on the presence of other virulence factors. Results of these two sets of tests proved the G3 strain to be phenotypically similar to the avirulent Rahman strain while antigenically identical to the virulent HM-1:IMSS, apart from the lack of the amoebapore-A protein. Intraperitoneal immunisation of hamsters with G3 trophozoites compared to sham immunised hamsters resulted in IgG anti-HM-1:IMSS antibodies. The level of humoral response was variable and further testing has to take place before introducing this new strain as a vaccine. (C) 2003 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.