Effects of hyperinsulinemia and hyperglycemia on lactate release and local blood flow in subcutaneous adipose tissue of healthy humans.
Effects of hyperinsulinemia and hyperglycemia on lactate release and local blood flow in subcutaneous adipose tissue of healthy humans.
复制标题
高胰岛素血症和高血糖对健康人皮下脂肪组织乳酸释放和局部血流的影响。
DOI：
10.1210/jcem.81.8.8768847
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
L. Tappy
中科院分区：
文献类型：
--
作者：
S. Henry;P. Schneiter;E. Jéquier;L. Tappy
为了确定高血糖症和高胰岛素血症对脂肪细胞产生乳酸的影响，在三个实验方案期间对健康志愿者进行了研究。在方案1中，口服葡萄糖后通过微透析（sc组织）测量间质乳酸浓度的变化。血浆乳酸浓度增加39.4 +/-6.0%，透析液乳酸浓度增加117.9 +/-16.3%。在方案2中，进行2.5小时高胰岛素正葡萄糖钳夹和生长抑素输注。血浆和透析液乳酸盐浓度分别增加27.1 +/- 5.5%和146.8 +/-44.5%。此外，通过探头输注[U-13 C]葡萄糖，透析液乳酸盐在13 C中富集，基础浓度过量2.5 +/- 0.3摩尔%，钳夹期间过量3.4 +/- 0.3摩尔%（与基础浓度相比，P < 0.05）。[13 C]尿素也通过探头输注，[13 C]尿素的流出与流入比用作局部血流的指标。在高胰岛素正葡萄糖钳夹结束时，它降低了10.2 +/- 3.6%（P < 0.001），表明血流量增加。在方案3中，在基础胰岛素浓度下进行高血糖钳夹（10.0 mmol/L）。它使透析液乳酸盐浓度增加43.5 +/- 15.9%，并且不改变血浆乳酸盐浓度或局部血流量。可以得出结论，高胰岛素血症，在较小程度上，高血糖刺激脂肪细胞中的葡萄糖转化为乳酸。高胰岛素血症，而不是高血糖症，也增加脂肪组织血流量。
To determine the effects of hyperglycemia and hyperinsulinemia on lactate production by adipocytes, healthy volunteers were studied during three experimental protocols. In protocol 1, the changes in interstitial lactate concentrations were measured by microdialysis (sc tissue) after oral glucose administration. The plasma lactate concentration increased by 39.4 +/- 6.0%, and the dialysate lactate concentration increased by 117.9 +/- 16.3%. In protocol 2, a 2.5-h hyperinsulinemic euglycemic clamp and somatostatin infusion were performed. The plasma and dialysate lactate concentrations increased by 27.1 +/- 5.5% and 146.8 +/- 44.5%, respectively. In addition, [U-13C]glucose was infused through the probe, and dialysate lactate was enriched in 13C at 2.5 +/- 0.3 molar percent excess basally and at 3.4 +/- 0.3 molar percent excess during the clamp (P < 0.05 vs. basal). [13C]Urea was also infused through the probe, and the outflow to inflow ratio of [13C]urea was used as an index of local blood flow. It decreased by 10.2 +/- 3.6% (P < 0.001) at the end of the hyperinsulinemic euglycemic clamp, indicating an increase in blood flow. In protocol 3, a hyperglycemic clamp (10.0 mmol/L) at the basal insulin concentration was performed. It increased the dialysate lactate concentration by 43.5 +/- 15.9% and did not alter the plasma lactate concentration or local blood flow. It is concluded that hyperinsulinemia and, to a lesser extent, hyperglycemia stimulate glucose conversion into lactate in adipocytes. Hyperinsulinemia, but not hyperglycemia, also increases adipose tissue blood flow.
影响因子：
15.9
作者：
JENSSEN, T;NURJHAN, N;GERICH, JE
通讯作者：
GERICH, JE
肾上腺素对人体全身和腿部肌肉胰岛素介导的葡萄糖摄取的影响：血流的作用。
DOI：
10.1152/ajpendo.1992.263.2.e199
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
Laakso,M;Edelman,SV;Brechtel,G;Baron,AD
通讯作者：
Baron,AD