Factors influencing the grass carp gut microbiome and its effect on metabolism
Factors influencing the grass carp gut microbiome and its effect on metabolism
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草鱼肠道微生物群的影响因素及其对代谢的影响
DOI：
10.1111/1574-6941.12256
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发表时间：
2014-03-01
影响因子：
4.2
通讯作者：
Zhang, Tanglin
中科院分区：
文献类型：
--
作者：
Ni, Jiajia;Yan, Qingyun;Zhang, Tanglin
肠道微生物区系因其在宿主新陈代谢、免疫和健康维持等方面的重要作用，近年来受到广泛关注。本研究对草鱼肠道微生物群落的影响因素进行了研究，并进一步探讨了肠道微生物群落对代谢的潜在影响。从24份肠道样本中筛选出43.39 Gb的元基因组序列，对其进行了全序列分析。共检测到1228个系统型(116个古生菌和1112个细菌)，其中大部分属于鞭毛门、变形杆菌和梭状杆菌。总共有41335的检测到的开放阅读框架(ORF)与京都百科全书的基因和基因组途径相匹配，从注释的ORF推断，碳水化合物和氨基酸代谢是主要的匹配途径。基于肠道微生物群落的系统发育组成和基因组成的冗余分析表明，肠道饱满度和摄食(即多花黑麦草与商业饲料，池塘养殖与野生)与肠道微生物群显著相关。此外，饲喂黑麦草的草鱼的肠道微生物群显著促进了碳水化合物、氨基酸和脂肪的许多生物合成和代谢途径。这些发现表明，草鱼肠道微生物群发挥的代谢作用可能会受到饲料的影响。这些发现有助于鱼类肠道微生物生态学领域的研究，也为后续的功能研究提供了依据。
Gut microbiota have attracted extensive attention recently because of their important role in host metabolism, immunity and health maintenance. The present study focused on factors affecting the gut microbiome of grass carp (Ctenopharyngodon idella) and further explored the potential effect of the gut microbiome on metabolism. Totally, 43.39Gb of screened metagenomic sequences obtained from 24 gut samples were fully analysed. We detected 1228 phylotypes (116 Archaea and 1112 Bacteria), most of which belonged to the phyla Firmicutes, Proteobacteria and Fusobacteria. Totally, 41335 of the detected open reading frames (ORFs) were matched to Kyoto Encyclopedia of Genes and Genomes pathways, and carbohydrate and amino acid metabolism was the main matched pathway deduced from the annotated ORFs. Redundancy analysis based on the phylogenetic composition and gene composition of the gut microbiome indicated that gut fullness and feeding (i.e. ryegrass vs. commercial feed, and pond-cultured vs. wild) were significantly related to the gut microbiome. Moreover, many biosynthesis and metabolism pathways of carbohydrates, amino acids and lipids were significantly enhanced by the gut microbiome in ryegrass-fed grass carp. These findings suggest that the metabolic role played by the gut microbiome in grass carp can be affected by feeding. These findings contribute to the field of fish gut microbial ecology and also provide a basis for follow-up functional studies.