Elevated levels of human collagenase inhibitor in blister fluids of diverse etiology.
Elevated levels of human collagenase inhibitor in blister fluids of diverse etiology.
复制标题
不同病因的水疱液中人胶原酶抑制剂的水平升高。
DOI：
10.1111/1523-1747.ep12455837
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Stricklin,GP
中科院分区：
文献类型：
--
作者：
Welgus,HG;Bauer,EA;Stricklin,GP
检查各种大疱性疾病的水疱液中是否存在人胶原酶抑制剂。一种免疫学上与人皮肤成纤维细胞产生的胶原酶抑制剂相同的蛋白质在不同病因的大疱内被发现处于高浓度。水泡液中胶原酶抑制剂的水平范围为0.9-12.5 μg/ml，平均为4.9μ g/ml。平均值比相应患者血清中的平均值高3- 4倍，超过血浆水平6- 8倍。使用加热和抽吸诱导的水疱研究了胶原酶抑制剂在水疱液中积累的时间过程。新形成的水泡中的浓度约为0.5 μg/ml，与血浆抑制剂水平几乎相同，并保持恒定约4小时。随后抑制剂浓度迅速升高，48 h后达到峰值，为1.6 μg/ml。我们推测，这种抑制剂在水疱液中的作用涉及抑制大疱腔内的活性蛋白酶，并可能限制水疱形成的程度或帮助伤口修复。
Blister fluids from a variety of bullous disorders were examined for the presence of human collagenase inhibitor. A protein immunologically identical to the collagenase inhibitor produced by human skin fibroblasts was found in high concentrations within bullae of diverse etiologies. Levels of collagenase inhibitor in blister fluids ranged from 0.9-12.5 μg/ml, averaging 4.9μ/ml. The mean values were 3- to 4-fold greater than those present in the sera of corresponding patients and exceeded plasma levels by 6- to 8-fold. The time course of collagenase inhibitor accumulation in blister fluid was studied using heat- and suction-induced bullae. The concentration in newly formed blisters was ˜0.5 μg/ml, virtually identical to plasma inhibitor levels, and remained constant for approximately 4 h. Inhibitor concentrations then rose rapidly, reaching peak values of ˜6 μg/ml after 48 h. We speculate that the role of this inhibitor in blister fluid involves the inhibitions of active proteinases within the bulla cavity and may occur to limit the extent of blister formation or to assist in wound repair.