THE INTERFERENCE OF TRUNCATED WITH NORMAL POTASSIUM CHANNEL SUBUNITS LEADS TO ABNORMAL-BEHAVIOR IN TRANSGENIC DROSOPHILA-MELANOGASTER
THE INTERFERENCE OF TRUNCATED WITH NORMAL POTASSIUM CHANNEL SUBUNITS LEADS TO ABNORMAL-BEHAVIOR IN TRANSGENIC DROSOPHILA-MELANOGASTER
复制标题
DOI：
10.1002/j.1460-2075.1989.tb08364.x
复制
发表时间：
1989-08-01
期刊：
影响因子：
11.4
通讯作者：
PONGS, O
中科院分区：
文献类型：
--
作者：
GISSELMANN, G;SEWING, S;PONGS, O
黑腹果蝇的Shaker基因座编码一个A型钾通道亚基家族。Sharker102突变体在基因剂量测试中表现为反形物。这种行为是由于产生截短的A通道亚基。我们认为它们通过形成多聚体A通道结构来干扰正常对应物的功能。通过构建携带编码截短的A型钾通道亚基的热诱导基因以及正常野生型剂量的A型钾通道亚基的转基因果蝇来测试这一假设。改变的亚基在幼虫、蛹或成虫阶段导致野生型转化为沙克尔蝇。转化苍蝇表现出热诱导的异常腿抖动行为和热诱导的促进神经递质释放幼虫神经肌肉接头。通过异常A通道亚单位的过表达，转基因D。黑胃可以以可预测的方式改变。
The Shaker locus of Drosophila melanogaster encodes a family of A-type potassium channel subunits. Sharker102 mutants behave as antimorphs in gene dosage tests. This behaviour is due to the production of truncated A-channel subunits. We propose that they interfere with the function of their normal counterpart by forming multimeric A-channel structures. This hypothesis was tested by constructing transgenic flies carrying a heat-inducible gene encoding a truncated A-type potassium channel subunit together with a normal wild type dosis of A-type potassium channel subunits. The altered subunit leads at larval, pupal or adult stages to the transformation of wild type into Shaker flies. The transformed flies exhibited a heat-inducible abnormal leg shaking behaviour and a heat-inducible facilitated neurotransmitter release at larval neuromuscular junctions. By the overexpression of an aberrant A-channel subunit the normal behaviour of transgenic D. melanogaster can be altered in a predictable way.