Alteration of ASIC1 expression in clear cell renal cell carcinom
Alteration of ASIC1 expression in clear cell renal cell carcinom
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DOI：
10.2147/ott.s86927
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发表时间：
2015-01-01
影响因子：
4
通讯作者：
Chen, Gang
中科院分区：
文献类型：
--
作者：
Li, Yan;Xu, Guoxiong;Chen, Gang
背景：酸性细胞外pH是肿瘤组织的主要特征。酸敏感离子通道 (ASIC) 代表变性蛋白/上皮 Na+ 通道家族的 H+ 门控亚组，由酸性微环境激活。关于 ASIC 在实体瘤中的表达和临床意义知之甚少。本研究的目的是检测ASIC1在人透明细胞肾细胞癌（CCRCC）中的表达，并确定ASIC1的表达是否与临床病理特征相关。方法：分别通过实时定量聚合酶链反应和Western blot分析检测CCRCC组织中ASIC1在mRNA和蛋白水平的表达。采用组织芯片评估75例CCRCC患者肿瘤组织和匹配的癌旁正常组织中ASIC1蛋白的表达。结果：在正常肾脏和CCRCC样本中检测到ASIC1表达。与匹配的正常肾组织相比，CCRCC 组织中 ASIC1 蛋白和 mRNA 的表达显着降低（P
Background: Acidic extracellular pH is a major feature of tumor tissue. Acid-sensing ion channels (ASICs) represent an H+-gated subgroup of the degenerin/epithelial Na+ channel family and are activated by acidic microenvironment. Little is known about the expression and clinical significance of ASICs in solid tumors. The purpose of this study was to examine the expression of ASIC1 in human clear cell renal cell carcinoma (CCRCC) and to determine if the expression of ASIC1 is associated with clinicopathological features.Methods: The expression of ASIC1 in CCRCC tissues at the mRNA and protein levels was determined by real-time quantitative polymerase chain reaction and Western blot analysis, respectively. A tissue microarray was used to assess the expression of ASIC1 protein in tumor tissue and matched adjacent normal tissues from 75 patients with CCRCC.Results: ASIC1 expression was detected in normal renal and CCRCC samples. The expressions of ASIC1 protein and mRNA were significantly decreased in the CCRCC tissues compared with matched normal renal tissues (P