Physical principles and visual-OMP software for optimal PCR design.
Physical principles and visual-OMP software for optimal PCR design.
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DOI：
10.1007/978-1-59745-528-2_1
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发表时间：
2007
影响因子：
--
通讯作者：
J. SantaLucia
中科院分区：
文献类型：
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作者：
J. SantaLucia
DNA杂交和折叠的物理原理在它们对设计最佳PCR的重要性的背景下被描述。描述了用于计算竞争性单分子和双分子物种浓度的多态平衡模型。明确提出了七个PCR设计的“神话”，并描述了适用于PCR设计的各种合适的物理模型。本章为理解聚合酶链式反应设计提供了理论框架，也为用户提供了实用指南。还描述了DNA Software，Inc.的Visual-OMP(寡核苷酸建模平台)程序包。
The physical principles of DNA hybridization and folding are described within the context of how they are important for designing optimal PCRs. The multi-state equilibrium model for computing the concentrations of competing unimolecular and bimolecular species is described. Seven PCR design “myths” are stated explicitly, and alternative proper physical models for PCR design are described. This chapter provides both a theoretical framework for understanding PCR design and practical guidelines for users. The Visual-OMP (oligonucleotide modeling platform) package from DNA Software, Inc. is also described.