Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.
Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.
复制标题
硅胶：寡核苷酸固相磷酸三酯合成的改进支持物。
DOI：
10.1093/nar/10.22.7439
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发表时间：
1982
影响因子：
14.9
通讯作者：
J. Lecocq
中科院分区：
文献类型：
--
作者：
V. Kohli;A. Balland;M. Wintzerith;R. Sauerwald;A. Staub;J. Lecocq
采用磷酸三酯法逐步合成脱氧寡核苷酸，使用HPLC级硅胶（Porasil B）作为固体支持物。该程序产生了用于合成低聚物的方便的流通系统，其中所有反应步骤（包括溴化锌脱三苯甲基方法）与所选择的载体相容。利用稳定的磷酸三酯中间体可以高产率合成长度为25-30个核苷酸的脱氧寡核苷酸。固体支持物的易于处理允许方便地合成混合的寡核苷酸序列。
The phosphotriester method for the stepwise synthesis of deoxyoligonucleotides has been employed using HPLC-grade silica gel (Porasil B) as the solid support. The procedure results in a convenient flow-through system for the synthesis of oligomers where all the reaction steps including the zinc bromide method of detritylation are compatible with the selected support. Deoxyoligonucleotides of 25-30 nucleotides in length can be synthesized in high yields utilising stable phosphotriester intermediates. Ease of handling of the solid support allows convenient synthesis of mixed oligonucleotide sequences.