Long non-coding ribonucleic acid zinc finger antisense 1 promotes the progression of colonic cancer by modulating ZEB1 expression
Long non-coding ribonucleic acid zinc finger antisense 1 promotes the progression of colonic cancer by modulating ZEB1 expression
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DOI：
10.1111/jgh.13646
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发表时间：
2017-06-01
影响因子：
4.1
通讯作者：
Yan, Dongwang
中科院分区：
文献类型：
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作者：
Fang, Changyi;Zan, Jianbao;Yan, Dongwang
背景与目的：长链非编码RNA锌指反义1 （ZFAS1）在肝细胞癌中频繁扩增，并通过增加锌指E-box结合同源盒1 （ZEB1）促进转移，从而促进上皮-间质转化（EMT）的进展。然而，ZFAS1在结肠癌中的表达模式和作用尚不清楚。本研究旨在探讨ZFAS1在结肠癌中的作用及其临床意义。方法：对73例结肠癌患者的配对临床组织标本及临床病理特征进行分析。采用实时定量聚合酶链反应法检测ZFAS1在结肠癌组织、细胞系和血浆中的表达水平。探讨了ZEB1和emt相关标志物的表达水平。通过细胞生物学实验探讨ZFAS1在调节细胞增殖和侵袭中的生物学作用，以及在调节EMT中的作用。结果：锌指反义1在结肠癌组织中与癌旁黏膜相比表达上调(P
Background and Aim: Long non-coding RNA zinc finger antisense 1 (ZFAS1) is frequently amplified in hepatocellular carcinoma and promotes metastasis by increasing zinc finger E-box binding homeobox 1 (ZEB1), which can potentiate the progression of epithelial-to-mesenchymal transition (EMT). However, the expression pattern and role of ZFAS1 in colonic cancer remains unknown. The present study aimed to investigate the role of ZFAS1 and its clinical significance in colonic cancer.Methods: Paired clinical colonic cancer tissue samples and clinicopathologic characteristics of 73 patients were analyzed. Quantitative real-time polymerase chain reaction analysis was used to evaluate expression levels of ZFAS1 in colonic cancer tissues, cell lines, and plasma. ZEB1 and EMT-related markers expression levels also were explored. Cell biology assays were used to explore the biologic consequences of ZFAS1 in regulating cell proliferation and invasion, as well as the roles in regulating EMT.Results: Zinc finger antisense 1 was up-regulated in colonic cancer tissues compared with adjacent mucosa (P