Effect of chronic wound exudates and MMP-2/-9 inhibitor on angiogenesis in vitro
Effect of chronic wound exudates and MMP-2/-9 inhibitor on angiogenesis in vitro
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DOI：
10.1097/01.prs.0000173447.81513.7a
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发表时间：
2005-08-01
影响因子：
3.6
通讯作者：
Pallua, N
中科院分区：
文献类型：
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作者：
Ulrich, D;Lichtenegger, F;Pallua, N
背景：新证据表明基质金属蛋白酶（MMP）可能促进血管生成以及产生血管生成抑制剂。在本研究中，在有或没有合成MMP-2/-9抑制剂的体外血管生成模型中检查了静脉功能不全性溃疡患者伤口渗出液的血管生成作用。方法：从20名静脉功能不全性溃疡患者和20名烧伤植皮后供区伤口的对照患者中获得伤口渗出液。血管生成模型中，无创面积液的5个孔用苏拉明(20μg/ml)作为阴性对照，另外5个孔用血管内皮生长因子(1μg/ml)作为阳性对照。对培养基中不含和含有浓度为2μM和20μM的合成MMP-2/-9抑制剂的慢性伤口液体进行分析。通过读图器计算小管的总长度。使用曼-惠特尼检验进行统计分析。显着性水平被认为是 p
Background: New evidence suggests that matrix metalloproteinases (MMPs) may facilitate angiogenesis as well as function to generate angiogenesis inhibitors. In this study, the angiogenic effect of wound exudates from patients with venous insufficiency ulcers was examined in an in vitro angiogenesis model with and without synthetic MMP-2/-9 inhibitor.Methods: Wound exudates were obtained from 20 patients with venous insufficiency ulcers and 20 control patients with donor-site wounds after skin grafting for burns. In the angiogenesis model, suramin (20 mu g/ml) was used in five wells without wound fluid as negative control, and vascular endothelial growth factor (1 mu g/ml) was used in five other wells as positive control. Chronic wound fluids were analyzed without and with a synthetic MMP-2/-9 inhibitor with a concentration of 2 mu M and 20 mu M in the medium. The total length of tubules was calculated by map reader. Statistical analysis was performed using the Mann-Whitney test. The level of significance was considered to be p