IL-10 and IL-27 producing dendritic cells capable of enhancing IL-10 production of T cells are induced in oral tolerance
IL-10 and IL-27 producing dendritic cells capable of enhancing IL-10 production of T cells are induced in oral tolerance
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DOI：
10.1016/j.imlet.2009.05.002
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发表时间：
2009-06-30
影响因子：
4.4
通讯作者：
Hachimura, Satoshi
中科院分区：
文献类型：
--
作者：
Shiokawa, Aya;Tanabe, Kosuke;Hachimura, Satoshi
口服耐受性是肠道免疫的一个关键特征，可产生对摄入抗原(Ag)的全身耐受性。树突状细胞(DC)被认为是重要的免疫调节因子，然而，DC在口服耐受诱导中的确切作用尚不清楚。在TCR转基因小鼠模型上，我们研究了口服Ag后小鼠脾、肠系膜淋巴结(MLN)和Peyer‘s Patch(PP)中树突状细胞的特征。耐受小鼠PP和MLN来源的DC可诱导共培养的T细胞产生IL-10，但不能表达Foxp3。给药5~7天后，IL-10的产生明显增加，尤以PP DC为甚。银染毒2~5天后，IL-27的产生明显增加。CD11b(+)DC较CD11b(-)DC显著表达IL-10和IL-27。这些结果表明，PP中产生IL-10和IL-27的DC通过与抗原特异性T细胞的相互作用而增加，这些DC在口服耐受中起到了诱导产生IL-10的T细胞的作用。(C)2009爱思唯尔B.V.保留所有权利
Oral tolerance is a key feature of intestinal immunity, generating systemic tolerance to ingested antigens (Ag). Dendritic cells (DC) have been revealed as important immune regulators, however, the precise role of DC in oral tolerance induction remains unclear. We investigated the characteristics of DC in spleen, mesenteric lymph node (MLN), and Peyer's patch (PP) after oral Ag administration in a TCR-transgenic mouse model. DC from PP and MLN of tolerized mice induced IL-10 production but not Foxp3 expression in cocultured T cells. IL-10 production was markedly increased after 5-7-day Ag administration especially in PP DC. On the other hand, IL-27 production was increased after 2-5-day Ag administration. CD11b(+) DC, which increased after ingestion of Ag, prominently expressed IL-10 and IL-27 compared with CD11b(-) DC. These results suggest that IL-10 and IL-27 producing DC are increased by interaction with antigen specific T cells in PP, and these DC act as an inducer of IL-10 producing T cells in oral tolerance. (C) 2009 Elsevier B.V. All rights reserved