Characterization of Two Novel Propachlor Degradation Pathways in Two Species of Soil Bacteria
Characterization of Two Novel Propachlor Degradation Pathways in Two Species of Soil Bacteria
复制标题
两种土壤细菌中两种新型丙草胺降解途径的表征
DOI：
10.1128/aem.65.2.802-806.1999
复制
发表时间：
1999
影响因子：
4.4
通讯作者：
E. Ferrer
中科院分区：
文献类型：
--
作者：
Margarita Martín;G. Mengs;J. Allende;Javier Fernández;R. Alonso;E. Ferrer
摘要丙草胺(2-氯-N-异丙基乙酰苯胺)是一种用于苗前除草的乙酰胺类除草剂。在这项研究中，我们分离并鉴定了一种土壤细菌，不动杆菌菌株BEM2，它能够利用这种除草剂作为唯一和有限的碳源。该菌株降解丙草胺的中间产物的鉴定和以前报道的假单胞菌(PEM1)降解丙草胺的新代谢物的表征支持两种不同的丙草胺降解途径。菌株PEM1与丙草胺积累的N-异丙基乙酰苯胺、乙酰苯胺、乙酰胺和邻苯二酚的洗涤细胞悬浮液。假单胞菌PEM1在丙草胺上的传代时间为3.4h，AKs为0.17±0.04mM，不动杆菌BEM2菌株在丙草胺上的传代时间为3.1h，Ks为0.3±0.07 mm。与菌株BEM2培养后，N-异丙基乙酰苯胺、N-异丙基苯胺、异丙胺和儿茶酚积累。这两条降解途径都是可诱导的，丙草胺环上碳原子的主要产物是二氧化碳。这些结果和已鉴定的代谢产物的生物降解实验表明，假单胞菌对丙草胺的代谢。菌株PEM1的代谢途径与不动杆菌的代谢途径不同。菌株BEM2。
ABSTRACT Propachlor (2-chloro-N-isopropylacetanilide) is an acetamide herbicide used in preemergence. In this study, we isolated and characterized a soil bacterium, Acinetobacter strain BEM2, that was able to utilize this herbicide as the sole and limiting carbon source. Identification of the intermediates of propachlor degradation by this strain and characterization of new metabolites in the degradation of propachlor by a previously reported strain ofPseudomonas (PEM1) support two different propachlor degradation pathways. Washed-cell suspensions of strain PEM1 with propachlor accumulated N-isopropylacetanilide, acetanilide, acetamide, and catechol. Pseudomonas strain PEM1 grew on propachlor with a generation time of 3.4 h and aKs of 0.17 ± 0.04 mM.Acinetobacter strain BEM2 grew on propachlor with a generation time of 3.1 h and a Ks of 0.3 ± 0.07 mM. Incubations with strain BEM2 resulted in accumulation of N-isopropylacetanilide,N-isopropylaniline, isopropylamine, and catechol. Both degradative pathways were inducible, and the principal product of the carbon atoms in the propachlor ring was carbon dioxide. These results and biodegradation experiments with the identified metabolites indicate that metabolism of propachlor by Pseudomonas sp. strain PEM1 proceeds through a different pathway from metabolism byAcinetobacter sp. strain BEM2.