Single-Cell Live Imaging
Single-Cell Live Imaging
复制标题
单细胞实时成像
DOI：
10.1007/978-1-4939-9240-9_24
复制
发表时间：
2019
期刊：
影响因子：
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通讯作者：
Toru Hiratsuka and Naoki Komatsu
中科院分区：
文献类型：
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作者：
Toru Hiratsuka and Naoki Komatsu
最近的荧光显微镜允许在各种目标中高通量获取5D （X， Y， Z， T和彩色）图像，例如培养细胞，3D球体/类器官，甚至具有单细胞分辨率的活组织。这项技术被认为有希望增加对生理和病理细胞表型异质性特征的认识，例如，癌细胞对抗癌药物治疗的不同反应。在这里，我们概述了微观应用，以捕获不同类型的目标的活细胞事件，以及几个概念的证明。2D实时成像将以基于fret的延时培养细胞成像为例，3D组织成像方案将辅以小鼠皮肤实时成像方法。
Recent fluorescence microscopy allows for high-throughput acquisition of 5D (X, Y, Z, T, and Color) images in various targets such as cultured cells, 3D spheroid/organoid, and even living tissue with single-cell resolution. The technology is considered promising to augment insights on heterogeneous features of both physiological and pathological cell phenotypes, for instance, distinct responses of cancer cells to anticancer drug treatment. Here we overview microscopic applications to capture live cell events for different types of targets, together with a couple of proof of concepts. The 2D live imaging will be exemplified by a FRET-based time-lapse cultured cell imaging, and 3D tissue imaging protocol will be complemented with a method for mouse skin live imaging.