Direct extracellular electron transfer for high electricity production by a new type of marine microalgae Nannochloropsis sp. HDY2
Direct extracellular electron transfer for high electricity production by a new type of marine microalgae Nannochloropsis sp. HDY2
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DOI：
10.1016/j.cej.2024.148636
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发表时间：
2024-01-19
影响因子：
15.1
通讯作者：
Liu，Fanghua
中科院分区：
文献类型：
--
作者：
Xie，Zhangzhang;Yang，Cuiyun;Liu，Fanghua
越来越多的自养微藻被发现具有细胞外电子转移（EET）能力。然而，微藻中EET途径的机制和功能尚未得到深入研究。本研究提出了一种新型海洋微藻Nannochloropsissp的直接胞外电子转移（DEET）的证据，令人印象深刻的电力生产。HDY 2在单室和双室光合微生物燃料电池（PFMCs）中的作用。Nannochloropsis sp.被光合抑制剂强烈抑制，表明参与微绿球藻胞外电子转移（EET）的电子。都来自光合作用。透析袋和上清液实验结果表明，微绿球藻（Nannochloropsis sp.）用避蚊胺发电在循环伏安法（CV）分析中缺乏典型的氧化还原峰和细胞周围的细胞外丝状组分（EFC）的出现支持这一发现。在强光照条件下，微绿球藻叶绿素a和类胡萝卜素含量均高于对照。迅速下降，而脂肪酸合成和发电量增加，表明DEET途径在微绿球藻抗光氧化中起着积极的作用。本研究首次证明了微拟球藻具有EET能力，并对DEET在微拟球藻环境适应中的积极作用提供了新的认识。
A growing number of autotrophic microalgae have been found to have extracellular electron transfer (EET) capabilities. However, the mechanism and function of EET pathway in microalgae have not thoroughly investigated. This study presents evidence of direct extracellular electron transfer (DEET) for impressive electricity production by a new type of marine microalgaeNannochloropsissp. HDY2 in the single and double-chamber photosynthetic microbial fuel cells (PFMCs). The electricity generation ofNannochloropsissp. is strongly inhibited by photosynthetic inhibitors, suggesting that electrons involved in the extracellular electron transfer (EET) ofNannochloropsissp. are derived from photosynthesis. The results of dialysis bag and supernatant experiments suggest thatNannochloropsissp. produce electricity by DEET. The lack of typical redox peaks in cyclic voltammetry (CV) analysis and the appearance of extracellular filamentous component (EFC) around cells support this finding. Under high light conditions, the chlorophyllaand carotenoid content ofNannochloropsissp. rapidly decreased while the fatty acid synthesis and electricity generation increased, suggesting that the DEET pathway play an active role in the resistance to photooxidation ofNannochloropsissp. This study provides the first evidence thatNannochloropsishas the capability of performing EET and sheds new light on the positive role of DEET on the environmental adaptation ofNannochloropsis.