Cyclic di-GMP: a second messenger required for long-term survival, but not for biofilm formation, in Mycobacterium smegmatis (Retracted article. See vol. 157, pg. 918, 2011)
Cyclic di-GMP: a second messenger required for long-term survival, but not for biofilm formation, in Mycobacterium smegmatis (Retracted article. See vol. 157, pg. 918, 2011)
复制标题
DOI：
10.1099/mic.0.2008/017806-0
复制
发表时间：
2008-10-01
期刊：
影响因子：
2.8
通讯作者：
Chatterji, Dipankar
中科院分区：
文献类型：
--
作者：
Kumar, Manish;Chatterji, Dipankar
C-di-GMP(c-di-GMP)在细菌适应环境变化中发挥重要作用。它协调各种途径，包括生物膜的形成、细胞表面的变化、宿主的定植和毒力。在这篇文章中，我们报告了污垢分枝杆菌中c-di-GMP的存在，以及它在有机体长期生存中的作用。污垢分枝杆菌是一种具有GGDEF和EAL结构域的双功能蛋白，在体外分别具有DGC和PDE-A活性。我们将该蛋白命名为MSDGC-1。MSDGC-1编码基因的缺失不会影响耻垢分枝杆菌的生长和生物膜的形成，但会影响在营养饥饿条件下的长期存活。大多数含有GGDEF和EAL结构域的蛋白质已被证明具有DGC或PDE-A活性。为了进一步了解该蛋白的调控，我们克隆了各个结构域，并测试了它们各自的活性。MSDGC-1是活性所必需的全长蛋白，因为它的GGDEF和EAL结构域在分离时是不活跃的。
Cyclic di-GMP (c-di-GMP) plays an important role in bacterial adaptation to enable survival in changing environments. It orchestrates various pathways involved in biofilm formation, changes in the cell surface, host colonization and virulence. In this article, we report the presence of c-di-GMP in Mycobacterium smegmatis, and its role in the long-term survival of the organism. M. smegmatis has a single bifunctional protein with both GGDEF and EAL domains, which show diguanylate cyclase (DGC) and phosphodiesterase (PDE)-A activity, respectively, in vitro. We named this protein MSDGC-1. Deletion of the gene encoding MSDGC-1 did not affect growth and biofilm formation in M. smegmatis, but long-term survival under conditions of nutritional starvation was affected. Most of the proteins that contain GGDEF and EAL domains have been demonstrated to have either DGC or PDE-A activity. To gain further insight into the regulation of the protein, we cloned the individual domains, and tested their respective activities. MSDGC-1, the full-length protein, is required for activity, as its GGDEF and EAL domains are inactive when separated.