Effects of perfluorinated compounds on development of zebrafish embryos
Effects of perfluorinated compounds on development of zebrafish embryos
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DOI：
10.1007/s11356-012-0977-y
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发表时间：
2012-08
影响因子：
5.8
通讯作者：
Xin-mei Zheng;Hongling Liu;W. Shi;Si Wei;J. Giesy;Hong-xia Yu
中科院分区：
文献类型：
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作者：
Xin-mei Zheng;Hongling Liu;W. Shi;Si Wei;J. Giesy;Hong-xia Yu
全氟化合物(PFC)已广泛应用于工业和消费品中，并在许多环境介质中频繁检测到。全氟辛烷磺酸(PFOS)、全氟辛酸(PFOA)和全氟农酸(PFNA)对小鼠、大鼠和水鸟的潜在生殖效应已有报道。全氟辛烷磺酸和全氟辛酸也被证实对斑马鱼胚胎产生毒物；然而，已报道的有效浓度是相互矛盾的。多氟烷基磷酸酯表面活性剂(包括FC807)是全氟辛烷磺酸和全氟辛酸的前体，但目前还没有关于FC807和全氟辛酸对斑马鱼胚胎影响的报道。因此，本研究旨在探讨这四种全氟化碳对斑马鱼胚胎发育的影响。选择正常受精斑马鱼胚胎，在24孔细胞培养板上分别加入不同浓度的全氟辛酸、全氟辛烷磺酸、全氟辛烷磺酸或FC807。使用数码相机和体视显微镜对胚胎的形态异常进行成像。通过配对观察胚胎受精后96h，记录胚胎存活率和畸形率。全氟碳化物以浓度依赖的方式引起致死性，潜在毒性大小顺序为全氟辛烷磺酸和全氟辛烷磺酸；FC807和GT；全氟辛烷磺酸和全氟辛烷磺酸。受精后48小时观察心包水肿和72或96-HPF脊柱扭曲畸形。全氟辛烷磺酸、全氟辛烷磺酸、全氟辛烷磺酸和FC807都在斑马鱼发育的早期阶段造成了结构异常。PFC均能延缓斑马鱼胚胎的发育。PFC的毒性与PFC链的长度和官能团有关。
Perfluorinated compounds (PFCs) have been widely used in industrial and consumer products and frequently detected in many environmental media. Potential reproductive effects of perfluorooctanesulfonate (PFOS), perfluorooctanoic acid (PFOA) and perfluorononanoic acid (PFNA) have been reported in mice, rats and water birds. PFOS and PFOA were also confirmed developing toxicants towards zebrafish embryos; however, the reported effect concentrations were contradictory. Polyfluorinated alkylated phosphate ester surfactants (including FC807) are precursor of PFOS and PFOA; however, there is no published information about the effects of FC807 and PFNA on zebrafish embryos. Therefore, this study was conducted to determine the effects of these four PFCs on zebrafish embryos. Normal fertilized zebrafish embryos were selected to be exposed to several concentrations of PFOA, PFNA, PFOS or FC807 in 24-well cell culture plates. A digital camera was used to image morphological anomalies of embryos with a stereomicroscope. Embryos were observed through matching up to 96-h post-fertilization (hpf) and rates of survival and abnormalities recorded. PFCs caused lethality in a concentration-dependent manner with potential toxicity in the order of PFOS > FC807 > PFNA > PFOA based on 72-h LC50. Forty-eight-hour post-fertilization pericardial edema and 72- or 96-hpf spine crooked malformation were all observed. PFOA, PFNA, PFOS and FC807 all caused structural abnormalities using early stages of development of zebrafish. The PFCs all retarded the development of zebrafish embryos. The toxicity of the PFCs was related to the length of the PFC chain and functional groups.