Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy.
Development and characterization of a scintillating cell imaging dish for radioluminescence microscopy.
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用于放射发光显微镜的闪烁细胞成像皿的开发和表征。
DOI：
10.1039/c8an00106e
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Pratx,Guillem
中科院分区：
文献类型：
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作者：
Sengupta,Debanti;Kim,TaeJin;Almasi,Sepideh;Miller,Stuart;Marton,Zsolt;Nagarkar,Vivek;Pratx,Guillem
放射性发光显微镜是一种新兴的模式，可用于成像放射性核素探针与微米级的分辨率。该技术作为探测单细胞的代谢行为以及筛选和表征放射性药物的方式特别有用，但是图像的质量严重依赖于用于对细胞成像的闪烁体材料。在本文中，我们详细介绍了一个显微镜皿的薄膜蒸发材料，Lu2O3：Eu，可用作未来消费品的蓝图。在开发了一个简单的质量控制方法的基础上，长寿命的α和β源，我们表征各种薄膜闪烁体样品的放射性发光特性。我们发现大多数样品的性能一致，但也确定了一些不符合规格的样品，从而强调了在生物实验之前进行常规质量控制的必要性。此外，我们测试和量化材料的透明度，并证明透明度与厚度相关。最后，我们评估了材料的生物相容性，并表明显微镜皿可以产生活单细胞的放射性发光图像。
Radioluminescence microscopy is an emerging modality that can be used to image radionuclide probes with micron-scale resolution. This technique is particularly useful as a way to probe the metabolic behavior of single cells and to screen and characterize radiopharmaceuticals, but the quality of the images is critically dependent on the scintillator material used to image the cells. In this paper, we detail the development of a microscopy dish made of a thin-film scintillating material, Lu2O3:Eu, that could be used as the blueprint for a future consumable product. After developing a simple quality control method based on long-lived alpha and beta sources, we characterize the radioluminescence properties of various thin-film scintillator samples. We find consistent performance for most samples, but also identify a few samples that do not meet the specifications, thus stressing the need for routine quality control prior to biological experiments. In addition, we test and quantify the transparency of the material, and demonstrate that transparency correlates with thickness. Finally, we evaluate the biocompatibility of the material and show that the microscopy dish can produce radioluminescent images of live single cells.