Gut microbiota in multiple sclerosis: possible influence of immunomodulators.
Gut microbiota in multiple sclerosis: possible influence of immunomodulators.
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DOI：
10.1097/jim.0000000000000192
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发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Mowry EM
中科院分区：
文献类型：
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作者：
Cantarel BL;Waubant E;Chehoud C;Kuczynski J;DeSantis TZ;Warrington J;Venkatesan A;Fraser CM;Mowry EM
肠道细菌的差异已被描述为几种自身免疫性疾病。在这项探索性的初步研究中，我们比较了多发性硬化症患者和健康对照者的肠道细菌，并评估了醋酸格拉替雷默和维生素D治疗对微生物群的影响。受试者是健康的白人女性，有或没有复发缓解型多发性硬化症，维生素D不足。多发性硬化症患者未经治疗或服用醋酸格拉替默。受试者在基线和补充维生素D3 （5000 IU/天）90天后收集粪便。通过16S rRNA与DNA微阵列杂交来评估操作分类单位的丰度。虽然存在肠道细菌群落的重叠，但在多发性硬化症患者中，一些操作分类单位（包括Faecalibacterium）的丰度较低。醋酸格拉替默治疗的MS受试者与未治疗的受试者相比，其群落组成存在差异，包括拟杆菌科、粪杆菌、瘤胃球菌、乳酸杆菌科、梭状芽胞杆菌和其他梭状芽胞杆菌。与其他组相比，未经治疗的多发性硬化症患者在补充维生素D后，Akkermansia、Faecalibacterium和Coprococcus属的数量增加。虽然整体细菌群落相似，但在健康和多发性硬化症受试者之间具体的操作分类单位不同。醋酸格拉替默和维生素D的补充与微生物群的差异或变化有关。这项研究是探索性的，需要更大规模的研究来证实这些初步结果。
Differences in gut bacteria have been described in several autoimmune disorders. In this exploratory pilot study, we compared gut bacteria in multiple sclerosis patients and healthy controls and evaluated the influence of glatiramer acetate and vitamin D treatment on the microbiota. Subjects were otherwise healthy white women with or without relapsing-remitting multiple sclerosis who were vitamin D insufficient. Multiple sclerosis patients were untreated or were receiving glatiramer acetate. Subjects collected stool at baseline and after 90 days of vitamin D3 (5,000 IU/day) supplementation. The abundance of operational taxonomic units was evaluated by hybridization of 16S rRNA to a DNA microarray. While there was overlap of gut bacterial communities, the abundance of some operational taxonomic units, including Faecalibacterium, was lower in multiple sclerosis patients. Glatiramer acetate-treated MS subjects showed differences in community composition compared to untreated subjects, including Bacteroidaceae, Faecalibacterium, Ruminococcus, Lactobacillaceae, Clostridium, and Other Clostridiales. Compared to the other groups, untreated multiple sclerosis subjects had an increase in the Akkermansia, Faecalibacterium, and Coprococcus genera after vitamin D supplementation. While overall bacterial communities were similar, specific operational taxonomic units differed between healthy and multiple sclerosis subjects. Glatiramer acetate and vitamin D supplementation were associated with differences or changes in the microbiota. This study was exploratory, and larger studies are needed to confirm these preliminary results.