A design of fluorescent probes for superoxide based on a nonredox mechanism
A design of fluorescent probes for superoxide based on a nonredox mechanism
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DOI：
10.1021/ja047018k
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发表时间：
2005-01-12
影响因子：
15
通讯作者：
Itoh, N
中科院分区：
文献类型：
--
作者：
Maeda, H;Yamamoto, K;Itoh, N
O2-·的荧光检测是基于3通过亲核取代反应生成相应的荧光素4，该荧光过程对H2 O2、t-BuOOH、NaOCl、1 O2、HO·、NO·和ONOO-的O2-·具有特异性。此外，谷胱甘肽、细胞色素P450还原酶/NADPH和心肌黄酶/NADH对探针3（X = Y = F）的荧光过程影响较小，可用于检测佛波酯刺激中性粒细胞释放的O2-·，灵敏度较高。
Fluorometric detection of O2-•is performed based on desulfonylation of3to the corresponding fluoresceins4through nucleophilic substitution, and this fluorescing process is quite specific toward O2-•over H2O2,t-BuOOH, NaOCl,1O2, HO•, NO•, and ONOO-. Furthermore, effects of glutathione, cytochrome P450 reductase/NADPH, and diaphorase/NADH are relatively small on the fluorescing process of probe3with X = Y = F, which is useful to detect O2-•released from neutrophils stimulated by phorbol myristate acetate with satisfactory sensitivity.