Isolation of a chondroitin sulfate--dermatan sulfate proteoglycan from bovine aorta.
Isolation of a chondroitin sulfate--dermatan sulfate proteoglycan from bovine aorta.
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从牛主动脉中分离硫酸软骨素——硫酸皮肤素蛋白多糖。
DOI：
10.1016/0003-9861(75)90043-0
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发表时间：
1975
影响因子：
3.9
通讯作者：
G. Berenson
中科院分区：
文献类型：
--
作者：
K. Ehrlich;B. Radhakrishnamurthy;G. Berenson
Material containing proteoglycans was extracted from bovine aorta by the dissociative solvent 3.0mMgCl2. The proteoglycan that remained in solution at low ionic strength was purified by isopycnic CsCl centrifugation (ϱ, 1.75 – 1.89 g/ml). From the lower third of the gradient a proteoglycan was isolated which behaved as a homogeneous material when analyzed by the ultracentrifuge and by electrophoresis on cellulose acetate. The proteoglycan contained 12% protein, 21% uronic acid, and 28% hexosamine. Analyses by hyaluronidase digestion and gas-liquid chromatography of the polysaccharide moieties of the proteoglycan showed a composition of 56% chondroitin 6-sulfate, 20% chondroitin 4-sulfate, and 7% dermatan sulfate. A copolymeric structure for the polysaccharide of the proteoglycan is proposed.
