Vector competence of North American mosquitoes (Diptera: Culicidae) for West Nile virus
Vector competence of North American mosquitoes (Diptera: Culicidae) for West Nile virus
复制标题
DOI：
10.1603/0022-2585-38.2.130
复制
发表时间：
2001-03-01
影响因子：
2.1
通讯作者：
Jones, JW
中科院分区：
文献类型：
--
作者：
Turell, MJ;O'Guinn, ML;Jones, JW
我们评估了几种北美蚊子传播新引入的西尼罗河病毒（WN）的可能性。在最近的西尼罗河病毒爆发期间，在纽约市大都会地区、弗吉尼亚州阿萨提格岛野生动物保护区或从已建立的殖民地收集的蚊子被允许以感染西尼罗河病毒的鸡为食，这些鸡是从1999年爆发期间死亡的一只乌鸦中分离出来的。大约2周后对这些蚊子进行测试，以确定感染、传播和传播率。白纹伊蚊（Skuse）、atropalpus （Coquillett）和japonicus （Theobald）极易感染，传播性感染几乎所有个体均通过叮咬传播。中等易感的有淡纹库蚊和客纹伊蚊。美根伊蚊（Meigen Aedes）、埃及伊蚊（L.）和带喙伊蚊（Wiedemann Aedes）对感染的抵抗力相对较强，但接种了WN病毒的个体蚊虫确实通过叮咬传播病毒。受感染的雌性CN库蚊将WN病毒传播给1,618个F-1后代中的一个，表明该病毒可能垂直传播。除了实验室媒介能力外，宿主的摄食偏好、相对丰度和活动季节也决定了这些物种在传播西风病毒方面可能发挥的作用。
We evaluated the potential for several North American mosquito species to transmit the newly introduced West Nile (WN) virus. Mosquitoes collected in the New York City metropolitan area during the recent WN virus outbreak, at the Assateague Island Wildlife Refuge, VA, or from established colonies were allowed to feed on chickens infected with WN virus isolated from a crow that died during the 1999 outbreak. These mosquitoes were tested approximate to2 wk later to determine infection, dissemination, and transmission rates. Aedes albopictus (Skuse),Aedes atropalpus (Coquillett), and Aedes japonicus (Theobald) were highly susceptible to infection, and nearly all individuals with a disseminated infection transmitted virus by bite. Culex pipiens L, and Aedes sollicitans (Walker) were moderately susceptible. In contrast, Aedes vexans (Meigen),Aedes aegypti (L.), and Aedes taeniorhynchus (Wiedemann) were relatively refractory to infection, but individual mosquitoes inoculated with WN virus did transmit virus by bite. Infected female CN, pipiens transmitted WN virus to one of 1,618 F-1 progeny, indicating the potential for vertical transmission of this virus. In addition to laboratory vector competence, host-feeding preferences, relative abundance, and season of activity also determine the role that these species could play in transmitting WN virus.