Microbial communities in rare earth mining soil after in-situ leaching mining
Microbial communities in rare earth mining soil after in-situ leaching mining
复制标题
原地浸矿开采后稀土矿土壤中微生物群落
DOI：
10.1016/j.scitotenv.2020.142521
复制
发表时间：
2021-02-10
影响因子：
9.8
通讯作者：
Zhang, Tian C.
中科院分区：
文献类型：
--
作者：
Liu, Jingjing;Liu, Wei;Zhang, Tian C.
离子吸附型稀土矿普遍采用地浸开采技术，造成了严重的环境问题和矿区土壤生态系统的恶化。然而，我们对采矿作业对这些生态系统中微生物群的影响的了解目前非常有限。采用定量聚合酶链反应（qPCR）和Illumina高通量测序技术，对稀土矿区土壤中原核生物和氨氧化微生物的多样性和组成进行了研究。结果表明，地浸开采对矿区土壤微生物群落有显著影响。细菌、古菌和氨氧化古菌的丰度与离子型稀土元素呈显著负相关，而丰度变化相对稳定。稀土元素总量和铵态氮是古细菌群落结构的最强预测因子，有机质是古细菌群落结构变化的关键因子。绿弯菌、变形菌、酸菌和放线菌是最丰富的细菌门，古菌群落以Thaumarchaeota为主。系统发育分析表明，未分类的Thaumarchaeota和Crenarchaeota是AOA的优势类群。氨氧化细菌（AOB）的未检测和AOA的丰富表明古细菌而不是细菌是主要负责氨氧化在矿区土壤中。网络分析表明，微生物之间的积极相互作用可以提高它们对这种恶劣环境的适应性或抵抗力。本研究对稀土矿山开采后土壤中的原核生物群落和功能群进行了全面分析，并探讨了土壤微生物之间潜在的相互作用机制。(C)2020 Elsevier B.V.保留所有权利。
In-situ leaching technology is now widely used to exploit ion adsorption rare earth ore, which has caused serious environmental problems and deterioration of mining soil ecosystems. However, our knowledge about the influences of mining operation on the microbiota in these ecosystems is currently very limited. In this study, diversity and composition of prokaryote and ammonia-oxidizing microorganisms in rare earth mining soil after in-situ leaching practice were examined using quantitative Polymerase Chain Reaction (qPCR) and Illumina high-throughput sequencing. Results showed that in-situ leaching mining considerably impacted microbial communities of the mining soils. The abundances of bacterial, archaeal, and ammonia-oxidizing archaea (AOA) were significantly and negatively correlated with ionic rare earth elements (REEs), while their diversities were relatively stable. Total rare earth elements (TREEs) and ammonium were the strongest predictors of the bacterial community structure, and organic matter was the key factor predicting the variation in the archaeal community. Chloroflexi, Proteobacteria, Acidobacteria, and Actinobacteria were the most abundant bacterial phyla, and archaeal communities were dominated by Thaumarchaeota. Phylogenetic analysis indicated that unclassified Thaumarchaeota and Crenarchaeota were the predominant AOA groups. The non-detection of ammonia-oxidizing bacteria (AOB) and the abundance of AOA indicated that archaea rather than bacteria were predominantly responsible for ammonia oxidation in the mining soil. Network analysis demonstrated that positive interactions among microorganisms could increase their adaptability or resistance to this harsh environment. This study provides a comprehensive analysis of the prokaryotic communities and functional groups in rare earth mining soil after mining operation, as well as insight into the potential interactive mechanisms among soil microbes. (C) 2020 Elsevier B.V. All rights reserved.