Effects of high gravity on amphibian development.
Effects of high gravity on amphibian development.
复制标题
高重力对两栖动物发育的影响。
DOI：
10.2187/bss.20.40
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
K. Kashiwagi
中科院分区：
文献类型：
--
作者：
A. Kashiwagi;H. Hanada;Satomi Kawakami;H. Kubo;T. Shinkai;H. Fujii;K. Kashiwagi
为了阐明高重力环境对卵和胚胎发育的可能影响，将皱纹蛙和非洲爪哇受精卵和早期胚胎分别在2G、5G、7G和10G饲养到蝌蚪孵化阶段。结果表明：(1)高重力显著延缓囊胚期前开始处理的卵子和胚胎的发育，并导致两头畸形和小头畸形，提示高重力容易破坏动物-胚胎轴。另一方面，在原肠胚期之后开始处理的胚胎显示出看起来正常的摄食蝌蚪的数量显著增加。(2)超重条件下发育的胚胎和蝌蚪，其大脑、脊索和肌肉的发育和分化均受到抑制。(3)在高度重力处理的胚胎和蝌蚪中，过氧化氢解毒系统可能发育异常，这可能导致氧化应激，导致相当大的细胞损伤。
In order to clarify the possible effects of high gravity environments on eggs and developing embryos, Rana rugosa and Xenopus laevis fertilized eggs and early embryos were raised in 2 G, 5 G, 7 G and 10 G up to the hatched tadpole stage. The results showed that: (1) High gravity significantly retarded the development of eggs and embryos beginning treatment before the blastula stage and induced various abnormalities, including two heads and microcephally suggesting that high gravity is apt to disrupt the animal-vegital axis. On the other hand, embryos beginning treatment after the gastrula stage showed a striking increase in the number of normal-appearing feeding tadpoles. (2) Autopsy revealed that brains, notochords and muscles were reduced in development and differentiation for embryos and tadpoles developed in high gravity. (3) It seems likely that the system for hydrogen peroxide detoxification develops abnormally in high gravity-treated embryos and tadpoles, which probably results in oxidative stress, leading to considerable cell damage.