Are calcifying matrix vesicles in atherosclerotic lesions of cellular origin?
Are calcifying matrix vesicles in atherosclerotic lesions of cellular origin?
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DOI：
10.1007/s00395-006-0637-9
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发表时间：
2007-03-01
影响因子：
9.5
通讯作者：
Valenzuela, Stella M.
中科院分区：
文献类型：
--
作者：
Bobryshev, Yuri V.;Killingsworth, Murray C.;Valenzuela, Stella M.
近年来，基质囊泡在动脉钙化初始阶段的作用已得到认识。已从动脉粥样硬化动脉中分离出基质钙化囊泡，并研究了钙化囊泡的生化组成。尚未进行任何研究来检查基质囊泡的精细结构，以便可视化其在动脉中钙化的后续阶段的特征。在目前的工作中，采用了高分辨率超微结构分析，研究表明人类动脉粥样硬化病变中的基质囊泡是异质的，我们将其分为两种主要类型。 I型钙化囊泡由两个电子致密层包围的囊泡呈现，这些囊泡被发现对动脉粥样硬化病变中的原位钙化过程具有抵抗力。 II型基质囊泡由被几个电子致密层包围的囊泡呈现，这些囊泡被发现代表动脉粥样硬化病变中的钙化囊泡。 为了检验多层片包围的基质囊泡的钙化可能简单地作为物理化学过程发生的假设，独立于细胞调节，我们生产了多层脂质体并以类似于基质中发生的方式在体外诱导其钙化动脉粥样硬化病变中的原位囊泡。
Over recent years, the role of matrix vesicles in the initial stages of arterial calcification has been recognized. Matrix calcifying vesicles have been isolated from atherosclerotic arteries and the biochemical composition of calcified vesicles has been studied. No studies have yet been carried out to examine the fine structure of matrix vesicles in order to visualize the features of the consequent stages of their calcification in arteries. In the present work, a high resolution ultrastructural analysis has been employed and the study revealed that matrix vesicles in human atherosclerotic lesions are heterogeneous with two main types which we classified. Type I calcified vesicles were presented by vesicles surrounded by two electron-dense layers and these vesicles were found to be resistant to the calcification process in atherosclerotic lesions in situ. Type II matrix vesicles were presented by vesicles surrounded by several electron-dense layers and these vesicles were found to represent calcifying vesicles in atherosclerotic lesions.To test the hypothesis that calcification of matrix vesicles surrounded by multilayer sheets may occur simply as a physicochemical process, independently from the cell regulation, we produced multilamellar liposomes and induced their calcification in vitro in a manner similar to that occurring in matrix vesicles in atherosclerotic lesions in situ.