Anthocyanins contribute to fruit defense against postharvest green mold
Anthocyanins contribute to fruit defense against postharvest green mold
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DOI：
10.1016/j.postharvbio.2021.111661
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发表时间：
2021-07-26
影响因子：
7
通讯作者：
Li, Zhengguo
中科院分区：
文献类型：
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作者：
Lin, Yunlong;Fan, Liqin;Li, Zhengguo
花色素苷是一类重要的植物次生代谢产物，具有保护植物免受逆境胁迫的作用。然而，与植物对非生物胁迫的抗性相比，花青素在植物对生物胁迫的抗性中的贡献知之甚少。在这里，我们的特点是花色素苷在保护水果免受绿色霉菌，柑橘果实采后的主要疾病的功能。与其他橙子相比，'塔罗科'橙子是富含花色苷的最重要的血橙之一，对引起柑橘采后绿色霉菌的坏死性真菌指状青霉（Pd）的敏感性降低。感染疫霉诱导了“塔罗科”橙子花色素苷的积累，外源处理显著降低了柑橘果实对疫霉的敏感性。活性氧的积累，这被证明有助于植物感染坏死营养型真菌病原体，在'塔罗科'橙子是低于其他柑橘后，Pd感染。外源花青素处理后，柑橘果实中的活性氧含量和相关基因的表达下降后，Pd感染。此外，转录组分析表明，许多基因可能参与了植物与病原菌的互作，“塔罗科”橙子在感染Pd后花色素苷的合成显著上调。这些发现突出了花青素对水果抗病性的贡献，并为柑橘采后绿色霉菌的控制提供了重要的见解。
Anthocyanins, an important class of pigmented and health-promoting plant secondary metabolites, are involved in protecting plants from stresses. However, the contribution of anthocyanins in plant resistance to biotic stresses is poorly understood compared with that in plant resistance to abiotic stresses. Here, we characterized the function of anthocyanins in protecting fruit from green mold, the major postharvest disease of citrus fruit. Compared with other oranges, 'Tarocco' orange, one of the most important blood oranges enriched in anthocyanins, showed reduced susceptibility to the necrotrophic fungus Penicillium digitatum (Pd) which causes citrus postharvest green mold. Pd infection induced the accumulation of anthocyanins in 'Tarocco' orange and exogenous treatment of anthocyanins significantly reduced the susceptibility of citrus fruit to Pd. The accumulation of reactive oxygen species (ROS), which was shown to contribute to plant infection of necrotrophic fungal pathogens, in 'Tarocco' orange was less than that in other oranges upon Pd infection. ROS content and associated gene expression in citrus fruit upon Pd infection were reduced after exogenous treatment of anthocyanins. Moreover, transcriptome analysis showed that a lot of genes probably participated in plant-pathogen interaction and anthocyanin biosynthesis were noticeably up-regulated in 'Tarocco' orange upon Pd infection. These findings highlight the contribution of anthocyanins to fruit disease resistance and provide significant insights into the control of citrus postharvest green mold.