Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics
Parallel on-axis holographic phase microscopy of biological cells and unicellular microorganism dynamics
复制标题
DOI：
10.1364/ao.49.002872
复制
发表时间：
2010-05-20
期刊：
影响因子：
1.9
通讯作者：
Wax, Adam
中科院分区：
文献类型：
--
作者：
Shaked, Natan T.;Newpher, Thomas M.;Wax, Adam
我们应用基于平行两步移相轴干涉术的宽视场定量相显微镜技术来观察活的生物细胞和微生物动力学。与以前的离轴方法相比，平行轴上全息方法在相机空间带宽消耗方面更有效，因此可以在给定特定数字相机的有限空间带宽的情况下捕获更精细的样本空间细节。此外，由于并行采集机制，该方法适用于可视化快速动态过程，允许干涉采集率等于相机帧速率。通过神经元和单细胞微生物的相显微镜实验证明了该方法。(C) 2010年美国光学学会
We apply a wide-field quantitative phase microscopy technique based on parallel two-step phase-shifting on-axis interferometry to visualize live biological cells and microorganism dynamics. The parallel on-axis holographic approach is more efficient with camera spatial bandwidth consumption compared to previous off-axis approaches and thus can capture finer sample spatial details, given a limited spatial bandwidth of a specific digital camera. Additionally, due to the parallel acquisition mechanism, the approach is suitable for visualizing rapid dynamic processes, permitting an interferometric acquisition rate equal to the camera frame rate. The method is demonstrated experimentally through phase microscopy of neurons and unicellular microorganisms. (C) 2010 Optical Society of America