Soil microbial communities and their co-occurrence networks in response to long-term Pb–Zn contaminated soil in southern China
Soil microbial communities and their co-occurrence networks in response to long-term Pb–Zn contaminated soil in southern China
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DOI：
10.1007/s11356-022-23962-1
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发表时间：
2022-11
影响因子：
5.8
通讯作者：
Hongyan Xu;Bingqing Fu;Jiaqi Lei;Hui-juan Kang;Jun Wang;Xinhao Huang;F. Zhu
中科院分区：
文献类型：
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作者：
Hongyan Xu;Bingqing Fu;Jiaqi Lei;Hui-juan Kang;Jun Wang;Xinhao Huang;F. Zhu
采矿对周边环境造成极大的重金属污染，对土壤微生物群落构成威胁。灌浆剂对土壤微生物群落的影响不仅与其总量有关，而且与化学组分的分布有关。然而，化学组分对土壤微生物的影响及其相互作用在很大程度上仍不清楚。本文研究了中国南方长期铅锌渣污染区土壤样品的理化性质和细菌、真菌群落特征，分别取自对照区、轻度(L)污染区、中度(M)污染区和重度(H)污染区。结果表明，细菌和真菌的群落组成和结构受土壤有机质影响显著，而群落多样性受土壤有机质影响不显著。影响细菌和真菌群落的关键环境因子是pH，化学组分对其变化的影响大于总有机质量。方差划分分析（VPA）表明，真菌群落的变化主要受HM总量的驱动，而细菌群落的变化主要受土壤化学性质的驱动。共现网络表明，真菌网络的物种间相互作用比细菌网络少，但真菌网络更稳定，因为其关键类群数量更显著，正相关比例更低。这些结果表明，真菌群落可以作为hm污染状况的指示分类群，而特定的hm响应真菌种类，如asTriangularia mangenotii、Saitozyma podzolica、cladosporium endophytica和genusrhizophagusa，由于它们在污染地区的相对丰度较低，可以被认为是相关的生物指标。此外，由于在污染水平下的相对丰度较高，研究发现对HM敏感的细菌OTUs有5个属，分别为：insulfurustis、Thiobacillus、Sphingomonas、Qipengyuania和sulphirhabduss，它们对HM胁迫具有耐受性。
Mining causes extreme heavy metal (HM) contamination to surrounding environments and poses threats to soil microbial community. The effects of HMs on soil microbial communities are not only related to their total amounts but also associated with the distribution of chemical fractions. However, the effects of chemical fractions on soil microbes and their interactions remain largely unclear. Here we investigated soil physicochemical properties and bacterial and fungal communities of soil samples from the control area and lightly (L), moderately (M), and heavily (H) contaminated areas, respectively, which were collected from long-term Pb–Zn slag contamination area in the southern China. The results showed that bacterial and fungal community composition and structure were significantly affected by HMs, while community diversity was not significantly affected by HMs. The critical environmental factor affecting bacterial and fungal communities was pH, and the impacts of chemical fractions on their changes were more significant than the total amounts of HMs. Variance partitioning analysis (VPA) revealed fungal community changes were mostly driven by HM total amounts, but bacterial community changes were mostly driven by soil chemical properties. Co-occurrence network indicated that interactions among species of fungal network were sparser than that of bacterial network, but fungal network was more stable, due to a more significant number of keystone taxa and a lower percentage of positive associations. These illustrated that the fungal community might serve as indicator taxa for HM-contaminated status, and specific HM-responsive fungal species such asTriangularia mangenotii,Saitozyma podzolica, andCladosporium endophytica, and genusRhizophaguscan be considered relevant bioindicators due to their less relative abundance in contaminated areas. Additionally, HM-responsive bacterial OTUs representing five genera withinSulfurifustis,Thiobacillus,Sphingomonas,Qipengyuania, andSulfurirhabduswere found to be tolerant to HM stress due to their high relative abundance in contaminated levels.