Comparison of human dental follicle cells and human periodontal ligament cells for dentin tissue regeneration.
Comparison of human dental follicle cells and human periodontal ligament cells for dentin tissue regeneration.
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DOI：
10.2217/rme.15.21
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发表时间：
2015-05
影响因子：
2.7
通讯作者：
Ye Tian;D. Bai;W. Guo;Jie Li;Jin Zeng;Longqiang Yang;Zongting Jiang;Lian Feng;Mei Yu;W. Tian
中科院分区：
文献类型：
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作者：
Ye Tian;D. Bai;W. Guo;Jie Li;Jin Zeng;Longqiang Yang;Zongting Jiang;Lian Feng;Mei Yu;W. Tian
目的比较人牙囊细胞(DFCs)和牙周膜细胞(PDLCs)的成牙潜能。材料与方法比较DFCs和PDLCs的体外和体内特性。分别与处理后的牙本质基质支架复合后，将DFCs和PDLCs植入小鼠背部皮下8周。结果蛋白质组学分析表明，DFCs和PDLCs中存在32个差异表达蛋白质。对收获的移植物的检测表明，PDLCs可以像DFCs一样形成牙本质样组织。然而，DFCs生成的牙本质组织结构更完整。结论PDLCs在牙本质基质诱导微环境中可促进牙本质样组织的再生。DFC比PDLCs表现出更显著的牙本质形成能力。
AIM To compare the odontogenic potential of human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs). MATERIALS & METHODS In vitro and in vivo characterization studies of DFCs and PDLCs were performed comparatively. DFCs and PDLCs were subcutaneously implanted into the dorsum of mice for 8 weeks after combined with treated dentin matrix scaffolds respectively. RESULTS Proteomic analysis identified 32 differentially expressed proteins in DFCs and PDLCs. Examination of the harvested grafts showed PDLCs could form the dentin-like tissues as DFCs did. However, the structure of dentin tissues generated by DFCs was more complete. CONCLUSION PDLCs could contribute to regenerate dentin-like tissues in the inductive microenvironment of treated dentin matrix. DFCs presented more remarkable dentinogenic capability than PDLCs did.