Transcriptional regulation of sorghum defense determinants against a phloem-feeding aphid
Transcriptional regulation of sorghum defense determinants against a phloem-feeding aphid
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DOI：
10.1104/pp.103.028324
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发表时间：
2004-01-01
期刊：
影响因子：
7.4
通讯作者：
Koiwa, H
中科院分区：
文献类型：
--
作者：
Zhu-Salzman, K;Salzman, RA;Koiwa, H
当被韧皮部取食的蚜虫（Schizaphis graminum）攻击时，高粱（Sorghum bicolor）激活茉莉酸（JA）和水杨酸（SA）调节的基因，以及已知的创伤和SA信号通路之外的基因。通过差异消减法从被蚜虫侵染的高粱幼苗和未被蚜虫侵染的高粱幼苗中获得了672个cDNA。随后的表达谱使用DNA微阵列和Northern印迹分析确定了82个转录本类型从这个集合响应于绿虫喂养，茉莉酸甲酯（MeJA），或SA应用。DNA序列分析表明，这些编码蛋白在直接防御，防御信号，氧化爆发，次生代谢，非生物胁迫，细胞维护和光合作用，以及未知功能的蛋白。在响应昆虫取食，高粱增加了许多防御基因的转录丰度，与一些SA依赖的病程相关的基因响应青虫更强烈比SA。相比之下，只有微弱的诱导MeJA调节防御基因后，绿虫治疗观察。然而，侵染试验证实，JA调节的途径在植物防御绿蝽中是有效的。某些转录激活专门由绿蝽侵扰观察，并可能代表独特的信号转导事件独立的JA和SA调节的途径。结果表明，植物协调调节防御基因的表达时，韧皮部喂养蚜虫攻击，但也表明，蚜虫是能够避免触发激活一些其他潜在的有效的植物防御机制，可能通过其特定的模式喂养。
When attacked by a phloem-feeding greenbug aphid (Schizaphis graminum), sorghum (Sorghum bicolor) activates jasmonic acid (JA)- and salicylic acid (SA)-regulated genes, as well as genes outside known wounding and SA signaling pathways. A collection of 672 cDNAs was obtained by differential subtraction with cDNAs prepared from sorghum seedlings infested by greenbug aphids and those from uninfested seedlings. Subsequent expression profiling using DNA microarray and northern-blot analyses identified 82 transcript types from this collection responsive to greenbug feeding, methyl jasmonate (MeJA), or SA application. DNA sequencing analyses indicated that these encoded proteins functioning in direct defense, defense signaling, oxidative burst, secondary metabolism, abiotic stress, cell maintenance, and photosynthesis, as well as proteins of unknown function. In response to insect feeding, sorghum increased transcript abundance of numerous defense genes, with some SA-dependent pathogenesis-related genes responding to greenbug more strongly than to SA. In contrast, only weak induction of MeJA-regulated defense genes was observed after greenbug treatment. However, infestation tests confirmed that JA-regulated pathways were effective in plant defense against greenbugs. Activation of certain transcripts exclusively by greenbug infestation was observed, and may represent unique signal transduction events independent of JA- and SA-regulated pathways. Results indicate that plants coordinately regulate defense gene expression when attacked by phloem-feeding aphids, but also suggest that aphids are able to avoid triggering activation of some otherwise potentially effective plant defensive machinery, possibly through their particular mode of feeding.