Growth promotion of transformed cells by iron in serum-free culture.
Growth promotion of transformed cells by iron in serum-free culture.
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在无血清培养物中铁促进转化细胞的生长。
DOI：
10.1093/carcin/6.3.355
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发表时间：
1985
期刊：
影响因子：
4.7
通讯作者：
Guy Vincendon
中科院分区：
文献类型：
--
作者：
Paul Basset;J. Zwiller;M. Revel;Guy Vincendon
在无血清培养基中加入铁或转铁蛋白可刺激C6胶质瘤和L1210白血病细胞的生长。当铁螯合在培养基中使用去铁胺时，转铁蛋白的生长促进作用丧失。L1210细胞可以在仅补充有转铁蛋白或FeCl 3的无血清培养基中连续生长。在后一种情况下，已经表明L1210细胞向培养基中分泌某种促进铁摄取的因子。L1210细胞在对数生长期的生长被去铁胺阻断在细胞周期的G1-S界面。转铁蛋白对细胞生长的作用也受到没食子酸丙酯的抑制，没食子酸丙酯是一种已知的防止脂质过氧化的抗氧化剂。铁的作用比氯化血红素更有效地逆转没食子酸丙酯对L1210细胞生长的影响。铁被发现激活纯化鸟苷酸环化酶的存在下，不饱和脂肪酸。这表明，环GMP合成可能参与铁促进转化细胞生长。
The growth of C6 glioma and L1210 leukemic cells has been stimulated in serum-free medium by the addition or iron or transferrin. The growth promoting action of transferrin was lost when iron was chelated in the culture medium using desferrioxamine. L1210 cells can be grown continuously in serum-free medium supplemented with transferrin or FeCl3 only. In this latter case, it has been shown that L1210 cells secrete into the medium some factor which facilitates iron uptake. The growth of L1210 cells in their exponential phase was blocked by desferrioxamine at the G1-S interface of the cell cycle. The action of transferrin on cell growth was also inhibited by propyl gallate - a known antioxidant which prevents lipid peroxidation. The action of iron was more potent than hemin in reversing the influence of propyl gallate on L1210 cell growth. Iron was found to activate purified guanylate cyclase in the presence of unsaturated fatty acids. This suggests that cyclic GMP synthesis could be involved in the promotion of transformed cell growth by iron.