Serum DNA binding activity in healthy subjects and in rheumatic disease.
Serum DNA binding activity in healthy subjects and in rheumatic disease.
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健康受试者和风湿病患者的血清 DNA 结合活性。
DOI：
10.1002/art.1780170110
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发表时间：
1974
影响因子：
--
通讯作者：
E. Leroy
中科院分区：
文献类型：
--
作者：
Peter Hasselbacher;E. Leroy
对 DNA 抗体法尔硫酸铵沉淀测定法的改进提高了灵敏度，并可检测健康受试者和患有除系统性红斑狼疮 (SLE) 之外的风湿病受试者的血清中的 DNA 结合。免疫化学纯 IgG 中结合的存在以及天然和变性 DNA 的抑制模式支持这样的论点：正常、非 SLE 和 SLE 血清的 DNA 结合是抗体，并且在种类上相似，但程度不同。正常和非 SLE 血清中 DNA 抗体的存在可能会限制这些抗体在 SLE 中的定性作用，并使 DNA 抗体与风湿性疾病中的抗球蛋白和其他抗核抗体相似。
Modifications of the Farr ammonium sulfate precipitation assay for DNA antibodies have increased sensitivity and led to the detection of DNA binding in the sera of healthy subjects and subjects with rheumatic disease other than systemic lupus erythematosus (SLE). The presence of binding in immunochemically pure IgG and the pattern of inhibition by native and denatured DNA support the thesis that DNA binding of normal, non-SLE, and SLE sera is antibody and similar in kind although not in degree. The presence of antibodies to DNA in normal and non-SLE sera may limit the qualitative role of these antibodies in SLE and render DNA antibodies similar to antiglobulin and other antinuclear antibodies in rheumatic disease.