Telomerase activity in 144 brain tumours.
Telomerase activity in 144 brain tumours.
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DOI：
10.1038/bjc.1998.267
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发表时间：
1998-05
影响因子：
8.8
通讯作者：
Kirino, T
中科院分区：
文献类型：
--
作者：
Sano, T;Asai, A;Mishima, K;Fujimaki, T;Kirino, T
永生化细胞的无限增殖被认为高度依赖于端粒酶的活性，端粒酶是一种将端粒重复合成到染色体末端的核糖核蛋白。用聚合酶链式反应端粒重复序列扩增法(TRAP)分析了99例良性脑肿瘤和45例恶性脑肿瘤的端粒酶活性。TRAP检测结果通过将每个标本的端粒酶活性与人脑胶质瘤细胞系T98G的端粒酶活性进行归一化来定量，以获得相对的端粒酶活性。根据放射自显影，端粒酶活性也被目测为阳性或阴性。端粒酶活性阴性的116例肿瘤的相对端粒酶活性为零，而端粒酶活性阳性的28例肿瘤的相对端粒酶活性为12-84.3%(平均为0%vs36.1+/-19.3%，P<；0.0001)。因此，端粒酶活性的量化证实了放射自显影上端粒酶活性的目视评估结果。根据评估，恶性脑瘤的端粒酶活性阳性率高于良性肿瘤(57.8%vs2.0%，P<；0.001)。这些数据表明，端粒酶活性阳性与恶性脑瘤密切相关，在良性肿瘤中相当罕见，如神经鞘瘤或脑膜瘤。
Unlimited proliferation in immortalized cells is believed to be highly dependent on the activity of telomerase, a ribonucleoprotein that synthesizes telomeric repeats onto chromosome ends. Using a polymerase chain reaction-based telomeric repeat amplification protocol (TRAP) assay, we analysed telomerase activity in 99 benign and 45 malignant brain tumours. The TRAP assay results were quantitated by normalizing the telomerase activity of each specimen to that of human glioma cell line T98G to obtain the relative telomerase activity. Telomerase activity was also assessed visually from the autoradiograms as being positive or negative. One hundred and sixteen tumours with negative telomerase activity had null relative telomerase activity, whereas 28 tumours with positive telomerase activity had relative telomerase activities of 12-84.3% (mean 0% vs 36.1 +/- 19.3%, P < 0.0001). Thus, quantification of telomerase activity confirmed the results of the visual evaluation of telomerase activity on autoradiograms. Based on the assessment, malignant brain tumours had a higher positive rate of telomerase activity than benign tumours (57.8% vs 2.0%, P < 0.001). These data indicate that positive telomerase activity is strongly associated with malignant brain tumours and is rather rare in benign tumours, such as neurinomas or meningiomas.