Exacerbated febrile responses to LPS, but not turpentine, in TNF double receptor-knockout mice.
Exacerbated febrile responses to LPS, but not turpentine, in TNF double receptor-knockout mice.
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在 TNF 双受体敲除小鼠中，LPS 的发热反应加剧，但松节油则不然。
DOI：
10.1152/ajpregu.1997.272.2.r563
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Kluger,MJ
中科院分区：
文献类型：
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作者：
Leon,LR;Kozak,W;Peschon,J;Kluger,MJ
我们观察了全身注射[脂多糖(LPS)，2.5 mg/kg或50 pg/kg ip]或局部(松节油，100微l sc)炎症刺激对肿瘤坏死因子(TNFR)双受体(TNFR)基因敲除小鼠的发热、运动能力、体重和摄食量的影响。与野生型小鼠相比，高剂量的内毒素基因敲除小鼠在发热早期(3~15h)表现为更严重的发热，而在发热后期(16~24h)和低剂量的内毒素引起的发热两组相似。在注射脂多糖后，两组小鼠的运动活动、体重和食物摄入量都有类似的减少。作为对松节油的反应，TNFR基因敲除小鼠和野生型小鼠对所有监测变量的反应几乎相同。这些结果提示：1)肿瘤坏死因子可剂量依赖性地调节小鼠对脂多糖的发热反应；2)肿瘤坏死因子对皮下注射松节油的发热无调节作用；3)基因敲除小鼠在调节对内毒素或皮下注射松节油的急性时相反应中可能存在细胞因子冗余。
We examined the effects of injections of systemic [lipopolysaccharide (LPS), 2.5 mg/kg or 50 pg/kg ip] or local (turpentine, 100 microl sc) inflammatory stimuli on fever, motor activity, body weight, and food intake in tumor necrosis factor (TNF) double receptor (TNFR)-knockout mice. A high dose of LPS resulted in exacerbated fevers in TNFR-knockout mice compared with wild-type mice for the early phase of fever (3-15 h); the late phase of fever (16-24 h) and fevers to a low dose of LPS were similar in both groups. Motor activity, body weight, and food intake were similarly reduced in both groups of mice after LPS administration. In response to turpentine, TNFR-knockout and wild-type mice developed virtually identical responses to all variables monitored. These results suggest that 1) TNF modulates fevers to LPS dose dependently, 2) TNF does not modulate fevers to a subcutaneous injection of turpentine, and 3) knockout mice may develop cytokine redundancy in the regulation of the acute phase response to intraperitoneally injected LPS or subcutaneously injected turpentine.