The Effect of Stress-Induced Senescence on Aging Human Cord Blood-Derived Endothelial Cells.
The Effect of Stress-Induced Senescence on Aging Human Cord Blood-Derived Endothelial Cells.
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DOI：
10.1007/s13239-013-0128-8
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发表时间：
2013-06-01
影响因子：
1.8
通讯作者：
Truskey, George A
中科院分区：
文献类型：
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作者：
Cheung, Tracy M;Ganatra, Mansi P;Truskey, George A
目得：我们试图确定应力诱导衰老对白蛋白的渗透性老化内皮祖细胞的影响。方法：人脐带血衍生的内皮细胞（hCB-ECs）和人主动脉内皮细胞（HAECs）处理与200 μ M H2 O2和FITC-牛血清白蛋白的渗透性进行了测定。随后用100 μ M 8-pCPT-2 ′-O-Me-cAMP处理一些样品，8-pCPT-2 ′-O-Me-cAMP是一种激活Epac 1-Rap 1途径的cAMP类似物。用EdU测定法测量细胞增殖。结果：hCB-ECs对H2 O2的通透性明显增加，但与HAECs的反应不同。低传代hCB-ECs的通透性增加约82%（p < 0.01），
PURPOSE: We sought to determine the effect of stress-induced senescence on the permeability to albumin of aging endothelial progenitor cells.METHODS: Human umbilical cord blood derived endothelial cells (hCB-ECs) and human aortic endothelial cells (HAECs) were treated with 200 muM H2O2 and permeability to FITC-bovine serum albumin was measured. Some samples were subsequently treated with 100muM 8-pCPT-2'-O-Me-cAMP, a cAMP analog that activates the Epac1-Rap1 pathway. Cell proliferation was measured with the EdU assay. Phase contrast, and immunofluorescence images were taken to observe morphological changes in cells after exposure to H2O2.RESULTS: hCB-ECs exposed to H2O2 exhibited a significant increase in permeability, but their response differed from the HAECs. Low passage hCB-ECs had a permeability increase of about 82% (p