Preventive effects of procyanidin A2 on glucose homeostasis, pancreatic and duodenal homebox 1, and glucose transporter 2 gene expression disturbance induced by bisphenol A in male mice.
Preventive effects of procyanidin A2 on glucose homeostasis, pancreatic and duodenal homebox 1, and glucose transporter 2 gene expression disturbance induced by bisphenol A in male mice.
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发表时间：
2016-04
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通讯作者：
A. Ahangarpour;G. Afshari;S. Mard;A. Khodadadi;M. Hashemitabar
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作者：
A. Ahangarpour;G. Afshari;S. Mard;A. Khodadadi;M. Hashemitabar
原花青素（PCs）是由儿茶素和表儿茶素分子形成的具有抗糖尿病特性的低聚化合物。双酚A（BPA）是食品和饮料包装中常用的化学材料。本研究旨在探讨原花青素A2 （PCA2）对BPA诱导的雄性小鼠葡萄糖稳态紊乱及胰腺和十二指肠homebox 1 （Pdx1）和葡萄糖转运蛋白2 （Glut2）基因表达的保护作用。首先在体外条件下，检测了5种浓度的PCA2 （3 ~ 300 μM）单独和联合BPA（100 μg/L）对离体胰岛胰岛素分泌的影响。接下来，利用流式细胞术检测BPA和PCA2对胰岛细胞凋亡的影响。在体内条件下，连续20 d给药BPA （100 μmol/kg）和PCA2 (10 μmol/kg)，检测血糖和胰岛素、Pdx1和Glut2基因表达及氧化应激标志物。结果表明，PCA2对双酚a诱导的胰岛细胞凋亡具有明显的抑制作用。BPA降低了胰腺组织中Pdx1和Glut2 mRNA的表达和抗氧化水平，而PCA2则阻止了这些作用。这些研究结果表明，使用富含PCA2的植物对高血糖和2型糖尿病有预防作用。
Procyanidins (PCs) as oligomeric compounds with antidiabetic properties formed from catechin and epicatechin molecules. Bisphenol A(BPA) is a common chemical material use in food and beverage packaging. The aim of this study was to explore the protective effects of procyanidin A2 (PCA2) against glucose homeostasis disturbance and gene expression of pancreatic and duodenal homebox 1 (Pdx1) as well as glucose transporter 2 (Glut2) induced by BPA in male mice. First tested these five concentrations of PCA2 (3 - 300 μM) alone and in combination with BPA(100 μg/L), on insulin secretion from isolated islets at in vitro condition. Next, examined the influence of BPA and PCA2 on islet apoptosis using flowcytometry. At in vivo condition, the BPA (100 μg/kg) and PCA2 (10 μmol/kg) administered for 20 days then, blood glucose and insulin, Pdx1 and, Glut2 genes expression, and oxidative stress markers examined. The results indicated that PCA2 strongly prevents islet cells apoptosis induced by BPA and, co-administration of PCA2 and BPA modified hyperglycemia. BPA reduced Pdx1 and Glut2 mRNA expression and antioxidant level in pancreas tissue, whereas PCA2 prevented from these effects. The findings from these studies suggest that use of PCA2 rich plants have preventive effects on hyperglycemia, and type 2 diabetes.