Molecular characterization and expression analysis of the Rab GTPase family in Vitis vinifera reveal the specific expression of a VvRabA protein
Molecular characterization and expression analysis of the Rab GTPase family in Vitis vinifera reveal the specific expression of a VvRabA protein
复制标题
DOI：
10.1093/jxb/ern132
复制
发表时间：
2008-06-01
影响因子：
6.9
通讯作者：
Tesniere, Catherine
中科院分区：
文献类型：
--
作者：
Abbal, Philippe;Pradal, Martine;Tesniere, Catherine
作为研究Rab GTPases是否参与葡萄果实发育的第一步，我们检索了Vitis vinifera EST和vrab家族成员的基因数据库。葡萄藤基因组被发现包含26个vvrab，这些vvrab可以分布在模式植物文献中描述的所有8个群体中。遗传作图成功；VvRabs大部分位于独立的染色体上，除了8个位于聚集在ChrUn_Random染色体上的基因组中尚未分配的部分。鉴定了vvrrab蛋白序列之间的保守区和发散区。采用实时定量RT-PCR分析11个vrrabs的转录物表达情况。除VvRabA5b外，在所有器官中均检测到转录本表达，但表达模式不同。在葡萄果实中，vvrrab转录本在果实发育的所有阶段都有表达，具有不同的表达谱，但A家族成员的表达谱一般相似。细胞培养中对生长调节剂的反应通常是针对每种vvrrab的，根据vvrrab，乙烯、生长素和脱落酸的表达模式不同。有趣的是，考虑到转录物的表达，使用针对AtRabA5c的单克隆抗体（ARA4）的western blotting显示，在所有7种被测试的红白莓品种中，成熟葡萄果实的外果皮中都有特异性表达。相比之下，在任何其他器官或组织中均未检测到表达。本文首次报道了葡萄球菌中Rab gtp酶的种类。本文结合文献资料讨论了一种特殊的vrrab在葡萄果实后期发育中的作用以及该Rab GTPase的潜在作用。
As a first step to investigate whether Rab GTPases are involved in grape berry development, the Vitis vinifera EST and gene databases were searched for members of the VvRab family. The grapevine genome was found to contain 26 VvRabs that could be distributed into all of the eight groups described in the literature for model plants. Genetic mapping was successfully performed; VvRabs were mostly located on independent chromosomes, apart from eight that were located on the as yet unassigned portions of the genome clustered in the ChrUn_Random chromosome. Conserved and divergent regions between VvRab protein sequences were identified. Transcript expression of 11 VvRabs was analysed by real-time quantitative RT-PCR. Except for VvRabA5b, transcript expression was detected, in general, in all the organs investigated, but with different patterns. In grape berries, VvRab transcripts were expressed at all stages of fruit development, with different profiles, except in the case of members of the A family which displayed generally similar patterns. The response to growth regulators in cell cultures was generally specific to each VvRab, with a differential pattern of expression for ethylene, auxin, and abscisic acid according to the VvRab. Interestingly, and unexpectedly considering transcript expression, western blotting using a monoclonal antibody raised against AtRabA5c (ARA4) showed a specific expression in the exocarp of ripe grape berries, in all seven red and white berry varieties tested. By contrast, no expression was detected in any of the other organs or tissues investigated. This paper contains the first description of Rab GTPases in V. vinifera. The involvement of a specific VvRab in grape berry late development and the potential role of this Rab GTPase are discussed in relation to literature data.