Biological assemblies provide novel templates for the synthesis of hierarchical structures and facilitate cell adhesion.
Biological assemblies provide novel templates for the synthesis of hierarchical structures and facilitate cell adhesion.
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DOI：
10.1002/adfm.200801215
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发表时间：
2008-12-22
影响因子：
19
通讯作者：
George, Anne
中科院分区：
文献类型：
--
作者：
Gajjeraman, Sivakumar;He, Gen;Narayanan, Karthikeyan;George, Anne
机械失配和植入物与自然组织环境之间缺乏相互作用是骨组织工程的主要缺陷。模拟骨基质的自组装过程和组成的生物材料将为构建具有新颖的骨修复和骨诱导性能的生物材料提供新的途径。在本研究中，我们采用生物启发策略设计了从头开始自组装的嵌合蛋白水凝胶，该水凝胶包含亮氨酸拉链基序和牙本质基质蛋白1结构域，该结构域被表征为矿化核因子。结果表明，该嵌合蛋白在拟生理缓冲液中可作为羟基磷灰石的核合剂，形成类似生物骨矿的高度定向磷灰石。它还可以作为成骨细胞黏附的诱导底物，促进细胞表面整合素的呈递和聚集，并调节局灶性接触的形成。这种具有骨传导和骨诱导双重性质的仿生“自下而上”结构应该会为骨组织工程开辟新的途径。机械失配和植入物与自然组织环境之间缺乏相互作用是骨组织工程的主要缺陷。模拟骨基质的自组装过程和组成的生物材料将为构建具有新颖的骨修复和骨诱导性能的生物材料提供新的途径。在本研究中，我们采用生物启发策略设计了从头开始自组装的嵌合蛋白水凝胶，该水凝胶包含亮氨酸拉链基序和牙本质基质蛋白1结构域，该结构域被表征为矿化核因子。结果表明，该嵌合蛋白在拟生理缓冲液中可作为羟基磷灰石的核合剂，形成类似生物骨矿的高度定向磷灰石。它还可以作为成骨细胞黏附的诱导底物，促进细胞表面整合素的呈递和聚集，并调节局灶性接触的形成。这种具有骨传导和骨诱导双重性质的仿生“自下而上”结构应该会为骨组织工程开辟新的途径。
Mechanical mismatch and the lack of interactions between implants and the natural tissue environment are the major drawbacks in bone tissue engineering. Biomaterials mimicking the self-assembly process and the composition of the bone matrix should provide new route for fabricating biomaterials possessing novel osteoconductive and osteoinductive properties for bone repair. In the present study, we employ bio-inspired strategies to design de novo self-assembled chimeric protein hydrogels comprising leucine zipper motifs flanked by dentin matrix protein 1 domain, which was characterized as a mineralization nucleator. Results showed that this chimeric protein could function as a hydroxyapatite nucleator in pseudo-physiological buffer with the formation of highly oriented apatites similar to biogenic bone mineral. It could also function as an inductive substrate for osteoblast adhesion, promote cell surface integrin presentation and clustering, and modulate the formation of focal contacts. Such biomimetic “bottom-up” construction with dual osteoconductive and osteoinductive properties should open new avenues for bone tissue engineering. Mechanical mismatch and the lack of interactions between implants and the natural tissue environment are the major drawbacks in bone tissue engineering. Biomaterials mimicking the self-assembly process and the composition of the bone matrix should provide new route for fabricating biomaterials possessing novel osteoconductive and osteoinductive properties for bone repair. In the present study, we employ bio-inspired strategies to design de novo self-assembled chimeric protein hydrogels comprising leucine zipper motifs flanked by dentin matrix protein 1 domain, which was characterized as a mineralization nucleator. Results showed that this chimeric protein could function as a hydroxyapatite nucleator in pseudo-physiological buffer with the formation of highly oriented apatites similar to biogenic bone mineral. It could also function as an inductive substrate for osteoblast adhesion, promote cell surface integrin presentation and clustering, and modulate the formation of focal contacts. Such biomimetic “bottom-up” construction with dual osteoconductive and osteoinductive properties should open new avenues for bone tissue engineering.
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影响因子：
5.1
作者：
Jérôme, V;Müller, R
通讯作者：
Müller, R
影响因子：
3.8
作者：
Ha, C;Park, CB
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Park, CB
影响因子：
41.2
作者：
He, G;Dahl, T;George, A
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影响因子：
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Mann, S