Degradation and solubilization of 13C-, 14C-side chain labeled synthetic lignin (dehydrogenative polymerizate) by laccase III of Coriolus versicolor
Degradation and solubilization of 13C-, 14C-side chain labeled synthetic lignin (dehydrogenative polymerizate) by laccase III of Coriolus versicolor
复制标题
云芝漆酶 III 对 13C-、14C-侧链标记的合成木质素（脱氢聚合产物）的降解和增溶
DOI：
10.1271/bbb.59.903
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
N. Morohoshi
中科院分区：
文献类型：
--
作者：
Y. Iimura;Y. Katayama;S. Kawai;N. Morohoshi
在典型的白色腐朽菌Coriolusversicolor的腐朽过程中，漆酶III是分泌量最大的酚氧化酶。在这项研究中，我们提出了一种可能的中间降解步骤的聚合木质素的纯化漆酶III使用合成的[β-13 C]和[β-14 C]木质素（DHP）。当[β-14 C]DHP与漆酶III孵育时，形成的水溶性降解产物约为所施用的[β-14 C]DHP的8%。漆酶III的酶攻击催化聚合木质素的侧链结构中的单体间键的裂解。在该菌的聚合木质素代谢中，漆酶活性与水溶性降解产物的积累密切相关。
During the decay of wood by the typical white rot fungus Coriolus versicolor, Laccase III was the most abundantly secreted phenol oxidase. In this study, we proposed a possibility of the intermediate degradation steps of polymeric lignin by a purified Laccase III using synthetic [β-13C] and [β-14C]lignin (DHP). When the [β-14C]DHP was incubated with Laccase III, the water-soluble degradation product formed was about 8% of the applied [β-14C]DHP. The enzymic attack of Laccase III catalyzed the cleavage of the intermonomer linkages in the side chain structure of the polymeric lignin. In polymeric lignin metabolism by this fungus, laccase activity was closely related to the accumulation of water-soluble degradation products.