Structural Mimics of Viruses Through Peptide/DNA Co-Assembly
Structural Mimics of Viruses Through Peptide/DNA Co-Assembly
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DOI：
10.1021/ja507833x
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发表时间：
2014-12-31
影响因子：
15
通讯作者：
Chau, Ying
中科院分区：
文献类型：
--
作者：
Ni, Rong;Chau, Ying
通过16-氨基酸肽和质粒DNA的协同共组装，构建了病毒结构的合成模拟物。这种短肽的合理设计，包括用于结合DNA和形成β折叠的片段，受到病毒衣壳蛋白的启发。由此产生的纳米结构，我们称之为纳米茧，表现为病毒样尺寸（65 × 47 nm）的椭圆体，并显示出类似4 nm宽的重复条纹。我们提出，共组装过程涉及DNA作为模板，以协助组织的肽链通过静电相互作用，而双层β-片层和它们的横向协会稳定的肽衣壳和组织内的DNA。这种层次结构提供了一个极其稳定的结构，保护肽和DNA免受酶消化。它开辟了一个新的和容易的途径来制造具有不同功能的病毒替代品。
A synthetic mimic of viral structure has been constructed by the synergistic co-assembly of a 16-amino acid peptide and plasmid DNA. The rational design of this short peptide, including segments for binding DNA and forming beta-sheet, is inspired by viral capsid protein. The resulting nanostructures, which we term nanococoons, appear as ellipsoids of virus-like dimension (65 x 47 nm) and display repeating stripes of similar to 4 nm wide. We propose that the co-assembly process involves DNA as a template to assist the organization of peptide strands by electrostatic interaction, while the bilayer beta-sheets and their lateral association stabilize the peptide capsid and organize the DNA within. The hierarchy affords an extremely stable structure, protecting peptide and DNA against enzymatic digestion. It opens a new and facile avenue to fabricate viral alternatives with diverse functions.