SEQUENTIAL STRUCTURAL RESPONSE OF LENS EPITHELIUM TO RETINA-CONDITIONED MEDIUM
SEQUENTIAL STRUCTURAL RESPONSE OF LENS EPITHELIUM TO RETINA-CONDITIONED MEDIUM
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DOI：
10.1016/0014-4835(84)90010-1
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发表时间：
1984-01-01
影响因子：
3.4
通讯作者：
MCAVOY, J
中科院分区：
文献类型：
--
作者：
WALTON, J;MCAVOY, J
当用视网膜条件培养基培养透镜上皮时，许多细胞在6-10天内经历纤维分化。在器官培养模型系统的结构性事件的时间序列的特点，这种变化进行了报道。在1小时内暴露于条件培养基，一些细胞退出上皮单层，并开始迁移到静止的细胞仍然附着在胶囊。到24小时，迁移活动主要是负责导致外植体成为多层，增加其厚度，同时减少其表面积。对照细胞通过微绒毛的相互指状排列沿沿着它们的侧边界彼此附着。BRCM处理后，微绒毛变平，膜有皱纹的外观。最终，随着培养的细胞发育成纤维，膜变直并形成球窝连接，并且它们的大量细胞器在自噬泡内发生降解。核仁开始扩大的16小时和时间的细胞已经暴露了24小时，一些核仁扩大到7倍，其原始面积，如在电子显微镜照片上测量。在接下来的几天里，随着核仁的变化，细胞质蛋白逐渐增加，细胞变得丰满或细长。在培养物中观察到的超微结构变化与在完整透镜中观察到的变化相似。这种保真度的变化表明，这种培养的透镜外植体系统是一个很好的模型，实验干预和分析的过程中涉及的终端透镜纤维分化。
When lens epithelium is cultured with retina-conditioned medium, many of the cells undergo fiber differentiation within 6-10 days. The temporal sequence of structural events that characterize this change in an organ culture model system were reported. Within 1 h of exposure to conditioned medium, some cells withdrew from the epithelial monolayer and began migrating over stationary cells still attached to the capsule. By 24 h, migratory activity was largely responsible for causing the explant to become multilayered, increasing its thickness while at the same time reducing its surface area. Control cells attached to each other along their lateral boundaries through interdigitations of microvilli. After BRCM treatment, microvilli flattened out and the membranes had a crinkled appearance. Eventually, as the cultured cells developed into fibers, membranes straightened and developed knob and socket junctions and large numbers of their organelles underwent degradation within autophagic vacuoles. Nucleoli began to enlarge by 16 h and by the time cells had been exposed for 24 h, some nucleoli were enlarged to 7-fold their original area, as measured on electron micrographs. This nucleolar change was followed over the next few days by a gradual increase in cytoplasmic protein, and cells became plump or elongated. The ultrastructural changes that were observed in culture are similar to those that can be seen in the intact lens. Such fidelity of change indicates that this cultured lens explant system is an excellent model for experimental intervention and analysis of the processes involved in terminal lens fibers differentiation.