ELEVATED SERUM LEVELS OF THE EOSINOPHIL GRANULE MAJOR BASIC-PROTEIN IN PATIENTS WITH EOSINOPHILIA
ELEVATED SERUM LEVELS OF THE EOSINOPHIL GRANULE MAJOR BASIC-PROTEIN IN PATIENTS WITH EOSINOPHILIA
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DOI：
10.1172/jci110080
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发表时间：
1981-01-01
影响因子：
15.9
通讯作者：
GLEICH, GJ
中科院分区：
文献类型：
--
作者：
WASSOM, DL;LOEGERING, DA;GLEICH, GJ
建立了人嗜酸性粒细胞主要碱性蛋白（MBP）的放射免疫分析法。105名正常对照患者血清中的平均MBP水平为454 ng/ml，而在188名患有各种疾病形式（包括嗜酸性粒细胞增多综合征）的患者样本中，测得的水平高达14，000 ng/ml。在正常人中血清MBP水平与嗜酸性粒细胞计数无关，但在嗜酸性粒细胞增多患者中呈正相关;嗜酸性粒细胞计数> 350 mm 3的患者通常显示MBP水平升高。许多皮肤病和正常嗜酸性粒细胞计数的患者血清MBP水平升高。MBP的分子量为9300，但在嗜酸性粒细胞增多症患者的血清中，MBP活性具有高分子量，> 50，000。通过Sephadex G-200和电聚焦的血清分析表明，MBP不是简单地聚合，而是结合到一个更大的载体分子。通过用二硫苏糖醇还原血清，碘乙酰胺烷基化，酸化至pH 2，然后在pH 2下用Sephadex G-50分级分离，可以从血清中分离单体MBP。在这些条件下，高达80%的MBP以单体形式出现。显然，嗜酸性粒细胞颗粒蛋白在血液中循环，与血清蛋白共价结合，并且在与嗜酸性粒细胞增多相关的一些疾病中存在升高的血清MBP浓度。
A radioimmunoassay was established for the human eosinophil granule major basic proein (MBP). The mean MBP level in sera from 105 normal control patients was 454 ng/ml, whereas in a sample of 188 patients with various disease forms, including the hypereosinophilic syndrome, levels as high as 14,000 ng/ml were measured. Serum MBP levels did not correlate with eosinophil counts in normal subjects, but a positive correlation was seen in patients with eosinophilia; the patients with eosinophil counts > 350 mm3 generally showed increased MBP levels. Many patients with skin disease and normal eosinophil counts had elevated serum MBP levels. Monomer MBP has a MW of 9300, but in sera of eosinophilia patients, the MBP activity was of high MW, > 50,000. Serum analyses by Sephadex G-200 and by electrofocusing suggest that MBP is not simply polymerized, but rather is bound to a larger carrier molecule. Monomeric MBP can be isolated from serum by reduction of serum with dithiothreitol, iodoacetamide alkylation, and acification to pH 2 followed by Sephadex G-50 fractionation at pH 2. Under these conditions, up to 80% of the MBP emerges in monomeric form. Apparently eosinophil granule proteins circulate in blood covalently bound to serum proteins, and elevated serum MBP concentrations are present in some diseases associated with eosinophilia.