Serum antibody responses to Pneumocystis carinii among different strains of normal and athymic mice
Serum antibody responses to Pneumocystis carinii among different strains of normal and athymic mice
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正常小鼠和无胸腺小鼠不同品系对卡氏肺孢子虫的血清抗体反应
DOI：
10.1128/iai.35.2.620-626.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
M. Rutledge
中科院分区：
文献类型：
--
作者：
P. D. Walzer;M. Rutledge
卡氏肺孢子虫感染在正常小鼠中通过施用皮质类固醇产生，在无胸腺小鼠中通过外源性小鼠或大鼠源性生物体的传播产生。通过间接荧光抗体技术测定，在六种正常小鼠中的五种中发现了卡氏肺孢子虫的血清抗体。虽然抗体滴度在对照组和类固醇治疗组小鼠之间差异很大，但它们与肺部卡氏肺孢子虫感染的强度成反比。在序贯研究中，类固醇给药期间抗体滴度较低，但随着类固醇停药而升高。抗体主要为免疫球蛋白G（IgG）类;也出现IgM抗体，但伊加抗体罕见。nu/nu小鼠很少产生抗卡氏肺孢子虫的血清抗体，尽管事实上一种菌株对外源性感染敏感而另一种菌株对外源性感染有抗性。nu/+小鼠产生针对大鼠和小鼠卡氏肺吸虫的特异性抗体，其主要是IgG。因此，宿主产生主要是IgG和T细胞依赖性的卡氏肺孢子虫抗体;然而，这些抗体似乎在卡氏肺孢子虫感染的易感性或抗性中并不重要。
Pneumocystis carinii infection was produced in normal mice by the administration of corticosteroids and in athymic mice by the transmission of exogenous mouse- or rat-derived organisms. Serum antibodies to P. carinii, measured by an indirect fluorescent-antibody technique, were found in five of six strains of normal mice. Although antibody titers varied widely among the control and steroid-treated mice, they were inversely proportional to the intensity of P. carinii infection in the lungs. In sequential studies, antibody titers were low during steroid administration but rose with steroid withdrawal. Antibodies were mainly of the immunoglobulin G (IgG) class; IgM antibodies also occurred, but IgA antibodies were rare. nu/nu mice rarely produced serum antibodies to P. carinii despite the fact that one strain was sensitive and the other was resistant to exogenous infection. nu/+ mice produced specific antibodies to rat and mouse P. carinii which were primarily IgG. Thus, the host produces antibodies to P. carinii which are mainly IgG and T-cell dependent; yet, these antibodies do not appear to be important in susceptibility or resistance to P. carinii infection.