Continuing germ line integration of AKV proviruses during the breeding of AKR mice and derivative recombinant inbred strains
Continuing germ line integration of AKV proviruses during the breeding of AKR mice and derivative recombinant inbred strains
复制标题
AKV 原病毒在 AKR 小鼠及其衍生重组近交系繁殖过程中的持续种系整合
DOI：
10.1128/jvi.42.1.165-175.1982
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发表时间：
1982
影响因子：
5.4
通讯作者：
R. Weinberg
中科院分区：
文献类型：
--
作者：
D. Steffen;B. Taylor;R. Weinberg
用Southern凝胶电泳法对小鼠白血病病毒AKV株的基因传播前病毒进行了分析。我们能够鉴定出AKR小鼠的限制性内切酶EcoRI片段，其中包含两个以前未识别的、通过基因传播的AKV前病毒。对AKR小鼠不同亚系的比较表明，它们的生殖系前病毒的补体具有相当大的异质性。这种异质性提供了AKR小鼠前病毒补体不稳定的证据。相反，在近亲交配期间，通过基因传播的前病毒的数量会增加。对C3H毒株的一系列亚系的检查表明，这种扩增依赖于病毒血症。我们估计，在病毒型小鼠中，每15到30年就会有一个新的前病毒固定在生殖系中。
The gel electrophoresis-hybridization technique of Southern was used to analyze genetically transmitted proviruses coding for the AKV strain of murine leukemia virus. We were able to identify the restriction endonuclease EcoRI fragments containing two previously unidentified, genetically transmitted AKV proviruses of AKR mice. Comparison of different sublines of AKR mice revealed considerable heterogeneity in their complement of germ line proviruses. This heterogeneity provides evidence that the provirus complement of AKR mice is not stable. Rather, the number of genetically transmitted proviruses increases during inbreeding. Examination of a series of sublines of the C3H strain indicated that this amplification is dependent on viremia. We estimate that, in viremic strains of mice, one new provirus becomes fixed in the germ line every 15 to 30 years.